Genome-Wide Identification of the Invertase Gene Family in Populus

Zhong Chen; Kai Gao; Xiaoxing Su; Pian Rao; Xinmin An

doi:10.1371/journal.pone.0138540

Genome-Wide Identification of the Invertase Gene Family in Populus

PLoS One. 2015 Sep 22;10(9):e0138540. doi: 10.1371/journal.pone.0138540. eCollection 2015.

Authors

Zhong Chen¹, Kai Gao¹, Xiaoxing Su², Pian Rao¹, Xinmin An¹

Affiliations

¹ National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of the Ministry of Education, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.
² Beijing Berry Genomics Company Limited, Beijing, China.

Abstract

Invertase plays a crucial role in carbohydrate partitioning and plant development as it catalyses the irreversible hydrolysis of sucrose into glucose and fructose. The invertase family in plants is composed of two sub-families: acid invertases, which are targeted to the cell wall and vacuole; and neutral/alkaline invertases, which function in the cytosol. In this study, 5 cell wall invertase genes (PtCWINV1-5), 3 vacuolar invertase genes (PtVINV1-3) and 16 neutral/alkaline invertase genes (PtNINV1-16) were identified in the Populus genome and found to be distributed on 14 chromosomes. A comprehensive analysis of poplar invertase genes was performed, including structures, chromosome location, phylogeny, evolutionary pattern and expression profiles. Phylogenetic analysis indicated that the two sub-families were both divided into two clades. Segmental duplication is contributed to neutral/alkaline sub-family expansion. Furthermore, the Populus invertase genes displayed differential expression in roots, stems, leaves, leaf buds and in response to salt/cold stress and pathogen infection. In addition, the analysis of enzyme activity and sugar content revealed that invertase genes play key roles in the sucrose metabolism of various tissues and organs in poplar. This work lays the foundation for future functional analysis of the invertase genes in Populus and other woody perennials.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Ascomycota / physiology
Chromosome Mapping
Chromosomes, Plant / genetics
Cold Temperature
Gene Expression Profiling
Gene Expression Regulation, Enzymologic / drug effects
Gene Expression Regulation, Plant / drug effects
Genome, Plant / genetics*
Host-Pathogen Interactions
Isoenzymes / genetics
Molecular Sequence Data
Multigene Family*
Phylogeny
Plant Leaves / enzymology
Plant Leaves / genetics
Plant Leaves / microbiology
Plant Proteins / genetics*
Plant Proteins / metabolism
Plant Roots / enzymology
Plant Roots / genetics
Plant Roots / microbiology
Populus / enzymology
Populus / genetics*
Populus / microbiology
Sequence Homology, Amino Acid
Sodium Chloride / pharmacology
beta-Fructofuranosidase / classification
beta-Fructofuranosidase / genetics*
beta-Fructofuranosidase / metabolism

Substances

Isoenzymes
Plant Proteins
Sodium Chloride
beta-Fructofuranosidase

Grants and funding

This work was supported by the Major State Basic Research Development Program of China (2012CB114505), the Fundamental Research Funds for the Central Universities (BLYJ201410), the Project of National Natural Science Foundation of China (31170631 and J1103516), and the National High Technology Research and Development Program of China (2013AA102703 and 2011AA100201). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. XS is an employee of Beijing Berry Genomics Company Limited. The funder provided support in the form of salary for XS, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section.