Large-Scale Nanoelectrode Arrays to Monitor the Dopaminergic Differentiation of Human Neural Stem Cells

Adv Mater. 2015 Nov 4;27(41):6356-62. doi: 10.1002/adma.201502489. Epub 2015 Sep 21.

Abstract

A novel cell-based biosensing platform is developed using a combination of sequential laser interference lithography and electrochemical deposition methods. This enables the sensitive discrimination of dopaminergic cells from other types of neural cells in a completely nondestructive manner. This platform and detection strategy may become an effective noninvasive in situ monitoring tool that can be used to determine stem cell fate for various regenerative applications.

Keywords: dopaminergic differentiation; electrochemical detection; large-scale nanopatterning; nanoelectrode arrays; neural stem cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biosensing Techniques
  • Cell Differentiation*
  • Dopamine / metabolism
  • Dopaminergic Neurons / cytology
  • Dopaminergic Neurons / metabolism*
  • Electrochemical Techniques*
  • Electrodes
  • Gold / chemistry
  • Humans
  • Levodopa / metabolism
  • Nanostructures / chemistry*
  • Neural Stem Cells / cytology
  • Neural Stem Cells / metabolism*
  • PC12 Cells
  • Rats
  • Tin Compounds / chemistry

Substances

  • Tin Compounds
  • Levodopa
  • indium tin oxide
  • Gold
  • Dopamine