Transcriptomic Profiling Reveals Complex Molecular Regulation in Cotton Genic Male Sterile Mutant Yu98-8A

PLoS One. 2015 Sep 18;10(9):e0133425. doi: 10.1371/journal.pone.0133425. eCollection 2015.

Abstract

Although cotton genic male sterility (GMS) plays an important role in the utilization of hybrid vigor, its precise molecular mechanism remains unclear. To characterize the molecular events of pollen abortion, transcriptome analysis, combined with histological observations, was conducted in the cotton GMS line, Yu98-8A. A total of 2,412 genes were identified as significant differentially expressed genes (DEGs) before and during the critical pollen abortion stages. Bioinformatics and biochemical analysis showed that the DEGs mainly associated with sugars and starch metabolism, oxidative phosphorylation, and plant endogenous hormones play a critical and complicated role in pollen abortion. These findings extend a better understanding of the molecular events involved in the regulation of pollen abortion in genic male sterile cotton, which may provide a foundation for further research studies on cotton heterosis breeding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Gene Expression Profiling
  • Gossypium / genetics*
  • Gossypium / metabolism
  • Phenotype
  • Reproduction
  • Transcriptome / genetics*

Grants and funding

Funding was provided by: 1. The Science-Technology Foundation for Outstanding Young Scientists of Henan Academy of Agricultural Sciences(Grant no. 2013YQ07). 2. The Programs for Science and Technology Development of Henan Province (Grant no. 122102110057). 3. The “863” Project of China (Grant No.2012AA101108-02-11). 4. National transgenic major projects of R. P. of China (2014ZX08005005-006).