A Novel Detection Platform for Shrimp White Spot Syndrome Virus Using an ICP11-Dependent Immunomagnetic Reduction (IMR) Assay

Bing-Hsien Liu; Yu-Chen Lin; Chia-Shin Ho; Che-Chuan Yang; Yun-Tsui Chang; Jui-Feng Chang; Chun-Yuan Li; Cheng-Shun Cheng; Jiun-Yan Huang; Yen-Fu Lee; Ming-Hung Hsu; Feng-Chun Lin; Hao-Ching Wang; Chu-Fang Lo; Shieh-Yueh Yang; Han-Ching Wang

doi:10.1371/journal.pone.0138207

A Novel Detection Platform for Shrimp White Spot Syndrome Virus Using an ICP11-Dependent Immunomagnetic Reduction (IMR) Assay

PLoS One. 2015 Sep 18;10(9):e0138207. doi: 10.1371/journal.pone.0138207. eCollection 2015.

Authors

Affiliations

¹ MagQu Co., Ltd., New Taipei City 231, Taiwan.
² Institute of Biotechnology, National Cheng Kung University, Tainan 701, Taiwan.
³ Institute of Bioinformatics and Biosignal Transduction, National Cheng Kung University, Tainan 701, Taiwan.
⁴ Graduate Institute of Translational Medicine, College of Medical Science and Technology, Taipei Medical University, Taipei 110, Taiwan.

Abstract

Shrimp white spot disease (WSD), which is caused by white spot syndrome virus (WSSV), is one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay to develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the most highly expressed WSSV protein). After characterizing the magnetic reagents (Fe3O4 magnetic nanoparticles coated with anti ICP11), the detection limit for ICP11 protein using IMR was approximately 2 x 10(-3) ng/ml, and the linear dynamic range of the assay was 0.1~1 x 10(6) ng/ml. In assays of ICP11 protein in pleopod protein lysates from healthy and WSSV-infected shrimp, IMR signals were successfully detected from shrimp with low WSSV genome copy numbers. We concluded that this IMR assay targeting ICP11 has potential for detecting the WSSV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animal Diseases / diagnosis
Animal Diseases / virology
Animals
Arthropod Proteins / immunology*
Arthropod Proteins / metabolism
Blotting, Western
Immunoprecipitation / methods*
Immunoprecipitation / veterinary
Limit of Detection
Magnetic Phenomena
Magnetite Nanoparticles* / chemistry
Penaeidae / virology*
Polymerase Chain Reaction / methods
Polymerase Chain Reaction / veterinary
Viral Envelope Proteins / analysis
Viral Envelope Proteins / genetics
Viral Envelope Proteins / immunology
Viral Envelope Proteins / metabolism
White spot syndrome virus 1 / genetics
White spot syndrome virus 1 / immunology
White spot syndrome virus 1 / isolation & purification
White spot syndrome virus 1 / metabolism*

Substances

Arthropod Proteins
Magnetite Nanoparticles
VP28 protein, white spot syndrome virus
Viral Envelope Proteins

Grants and funding

This work was supported by Agricultural Technology Research Institute in Taiwan (grant 103AG-a5.2-08). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. BHL, YCL, CSH, CCY, YTC, JFC, YFL, MHH, FCL and SYY are employed by MagQu Co. The funder provided support in the form of salaries for authors [BHL, YCL, CSH, CCY, JFC, YFL, MHH, FCL and SYY], but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. The specific roles of these authors are articulated in the 'author contributions' section.