Rapid Detection of Subtype H10N8 Influenza Virus by One-Step Reverse Transcription-Loop-Mediated Isothermal Amplification Methods

J Clin Microbiol. 2015 Dec;53(12):3884-7. doi: 10.1128/JCM.02165-15. Epub 2015 Sep 16.

Abstract

We developed hemagglutinin- and neuraminidase-specific one-step reverse transcription-loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and the assays did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity, and they can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chickens
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Influenza A Virus, H10N8 Subtype / genetics
  • Influenza A Virus, H10N8 Subtype / isolation & purification*
  • Influenza in Birds / diagnosis*
  • Influenza in Birds / virology
  • Neuraminidase / genetics
  • Nucleic Acid Amplification Techniques / methods*
  • Reverse Transcription*
  • Sensitivity and Specificity
  • Temperature
  • Time Factors
  • Viral Proteins / genetics

Substances

  • Hemagglutinin Glycoproteins, Influenza Virus
  • Viral Proteins
  • NA protein, influenza A virus
  • Neuraminidase