Abstract
We developed hemagglutinin- and neuraminidase-specific one-step reverse transcription-loop-mediated isothermal amplification assays for detecting the H10N8 virus. The detection limit of the assays was 10 copies of H10N8 virus, and the assays did not amplify nonspecific RNA. The assays can detect H10N8 virus from chicken samples with high sensitivity and specificity, and they can serve as an effective tool for detecting and monitoring H10N8 virus in live poultry markets.
Copyright © 2015, American Society for Microbiology. All Rights Reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Chickens
-
Hemagglutinin Glycoproteins, Influenza Virus / genetics
-
Influenza A Virus, H10N8 Subtype / genetics
-
Influenza A Virus, H10N8 Subtype / isolation & purification*
-
Influenza in Birds / diagnosis*
-
Influenza in Birds / virology
-
Neuraminidase / genetics
-
Nucleic Acid Amplification Techniques / methods*
-
Reverse Transcription*
-
Sensitivity and Specificity
-
Temperature
-
Time Factors
-
Viral Proteins / genetics
Substances
-
Hemagglutinin Glycoproteins, Influenza Virus
-
Viral Proteins
-
NA protein, influenza A virus
-
Neuraminidase