Heat-Induced Radiolabeling of Nanoparticles for Monocyte Tracking by PET

Angew Chem Int Ed Engl. 2015 Oct 26;54(44):13002-6. doi: 10.1002/anie.201505525. Epub 2015 Sep 14.

Abstract

Heat-induced radiolabeling (HIR) yielded (89) Zr-Feraheme (FH) nanoparticles (NPs) that were used to determine NP pharmacokinetics (PK) by positron emission tomography (PET). Standard uptake values indicated a fast hepatic uptake that corresponded to blood clearance, and a second, slow uptake process by lymph nodes and spleen. By cytometry, NPs were internalized by circulating monocytes and monocytes in vitro. Using an IV injection of HIR (89) Zr-FH (rather than in vitro cell labeling), PET/PK provided a view of monocyte trafficking, a key component of the immune response.

Keywords: heat-induced radiolabeling; imaging; monocyte trafficking; nanoparticles; pharmacokinetics; positron emission tomography.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Hot Temperature*
  • Metal Nanoparticles / chemistry*
  • Mice
  • Monocytes / cytology*
  • Positron-Emission Tomography*
  • Radioisotopes / chemistry
  • Radioisotopes / pharmacokinetics
  • Radiopharmaceuticals / chemistry
  • Radiopharmaceuticals / pharmacokinetics*
  • Tissue Distribution
  • Zirconium / chemistry
  • Zirconium / pharmacokinetics*

Substances

  • Radioisotopes
  • Radiopharmaceuticals
  • Zirconium