Rapid SERS monitoring of lipid-peroxidation-derived protein modifications in cells using photonic crystal fiber sensor

J Biophotonics. 2016 Jan;9(1-2):32-7. doi: 10.1002/jbio.201500168. Epub 2015 Sep 14.

Abstract

We proposed a side channel photonic crystal fiber (SC-PCF) based Surface enhanced Raman spectroscopy (SERS) platform which is able to accurately monitor lipid peroxidation derived protein modifications in cells. This platform incorporates linoleamide alkyne (LAA), which is oxidized and subsequently modifies proteins in cells with alkyne functional group upon lipid peroxidation. By loading the side channel of SC-PCF with a mixture of gold nanoparticles and LAA treated cells, and subsequently measuring the interference-free alkyne Raman peak from these proteins in cells, strong SERS signal was obtained. The platform provides a method for the rapid monitoring of lipid peroxidation derived protein modification in cells.

Keywords: HepG2 cell; Surface enhanced Raman spectroscopy; gold nanoparticles; linoleamide alkyne; lipid peroxidation; side channel PCF.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzene Derivatives / pharmacology
  • Biological Transport / drug effects
  • Hep G2 Cells
  • Humans
  • Linoleic Acids / metabolism
  • Lipid Peroxidation* / drug effects
  • Optical Fibers*
  • Photons*
  • Proteins / metabolism*
  • Spectrum Analysis, Raman / instrumentation*
  • Time Factors

Substances

  • Benzene Derivatives
  • Linoleic Acids
  • Proteins
  • linoleamide
  • cumene hydroperoxide