The ADAR1 editing enzyme is encapsidated into HIV-1 virions

Virology. 2015 Nov:485:475-80. doi: 10.1016/j.virol.2015.07.027. Epub 2015 Sep 9.

Abstract

Adenosine deaminase acting on RNA1 (ADAR1) was previously reported to affect HIV-1 replication. We report data showing that ADAR1 interacts with the HIV-1 p55 Gag protein, the major structural protein of the immature virus capsid. Furthermore, we found that the endogenous ADAR1 is incorporated into virions purified from the supernatant of primary HIV-1-infected CD4(+) T lymphocytes. Additional experiments demonstrated that the expression of the p55 Gag protein is sufficient for ADAR1 incorporation into virus-like particles (VLPs). Overall, our data originally support the evidence that ADAR1 can be part of the cell protein array uploaded in HIV-1 particles.

Keywords: ADAR1; Encapsidation; HIV-1; p55 Gag.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Deaminase / chemistry
  • Adenosine Deaminase / metabolism*
  • Cell Line
  • HIV-1 / physiology*
  • Humans
  • Protein Binding
  • Protein Interaction Domains and Motifs
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / metabolism*
  • Virion*
  • gag Gene Products, Human Immunodeficiency Virus / metabolism

Substances

  • RNA-Binding Proteins
  • gag Gene Products, Human Immunodeficiency Virus
  • ADAR protein, human
  • Adenosine Deaminase