Regulated expression of virulence gene mviN provides protective immunity and colonization control of Salmonella in poultry

Vaccine. 2015 Oct 5;33(41):5365-5370. doi: 10.1016/j.vaccine.2015.08.074. Epub 2015 Sep 4.

Abstract

Current live attenuated vaccines for control of Salmonella in poultry persist in the ceca and may persist in the environment. In this paper we report the construction and characterization of the vaccine efficacy of a Salmonella mutant strain with inducible mviN expression and rapid clearance from the host. The mutant was effective in oral immunization of the broiler chicken host against a virulent Salmonella oral challenge strain, having a mean 7×10(6)CFU/g in the ceca of unvaccinated controls compared to a mean 2×10(3)CFU/g in the ceca of vaccinated chickens at 4 weeks post-challenge (6 weeks of age). The mutant strain also demonstrated immunogenicity, reduced organ colonization, and rapid clearance in broiler chickens within 3 weeks of inoculation.

Keywords: Caco-2; HD-11; Poultry; Salmonella; Vaccine; murJ; mviN.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Bacterial / immunology
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / immunology*
  • Cell Line
  • Cell Survival
  • Chickens
  • Gene Expression Regulation, Bacterial*
  • Immunoglobulin G / immunology
  • Macrophages / immunology
  • Macrophages / metabolism
  • Macrophages / microbiology
  • Male
  • Mutation
  • Poultry
  • Salmonella / genetics*
  • Salmonella / immunology*
  • Salmonella Infections, Animal / immunology*
  • Salmonella Infections, Animal / microbiology
  • Salmonella Infections, Animal / prevention & control*
  • Time Factors
  • Vaccination / veterinary
  • Virulence / genetics

Substances

  • Antibodies, Bacterial
  • Bacterial Proteins
  • Immunoglobulin G