The impact of a number of synthetic nucleoside derivatives on the growth and survival of cultured human ovarian tumor cells (line SKOV-3) and normal human lung fibroblasts was investigated. It was shown that the dialdehyde derivatives of uridine, 1-β-D-eritrofuranozyl uracil and 3'-O-β-D-ribofuranosyl-2'-deoxythymidine, in contrast to their unoxidized counterparts, exert marked toxic effect on SKOV-3 cells. Cultured human fibroblasts were less susceptible to the damaging effect of the dialdehyde nucleosides. The dialdehyde derivative of 1-β-D-eritrofuranozyl uracil demonstrated greatest differences in the cytotoxic effect on these cultures: inhibition of tumor SKOV-3 cells growth on 50% or more was achieved at the concentrations of this compound ten times lower than in the case of normal fibroblasts.
Issledovano vliianie riada sinteticheskikh proizvodnykh nukleozidov na rost i vyzhivaemost' kul'tiviruemykh kletok opukholi iaichnika cheloveka (liniia SKOV-3) i kul'tiviruemykh normal'nykh fibroblastov legkikh cheloveka. Pokazano, chto dial'degidnye proizvodnye uridina, 1--D-éritrofuranoziluratsila i 3-O--D-ribofuranozil-2-dezoksitimidina, v otlichie ot ikh neokislennykh analogov, okazyvali vyrazhennoe toksicheskoe deĭstvie na kletki SKOV-3. Kul'tiviruemye fibroblasty cheloveka okazalis' menee vospriimchivymi k povrezhdaiushchemu deĭstviiu dial'degidnykh nukleozidov. Naibol'shie razlichiia v tsitotoksicheskom deĭstvii na éti kul'tury byli vyiavleny dlia dial'degidnogo proizvodnogo 1--D-éritrofuranoziluratsila: podavlenie rosta opukholevykh kletok SKOV-3 na 50% i bolee dostigalos' pri kontsentratsiiakh étogo soedineniia v desiatki raz men'shikh, chem v sluchae normal'nykh fibroblastov.
Keywords: SKOV3 human ovarian carcinoma cell line; antitumor action; cytotoxicity; dialdehyde derivatives of nucleosides; human fibroblasts.