The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine

G H M Sagor; Masataka Inoue; Dong Wook Kim; Seiji Kojima; Masaru Niitsu; Thomas Berberich; Tomonobu Kusano

doi:10.1016/j.febslet.2015.08.045

The polyamine oxidase from lycophyte Selaginella lepidophylla (SelPAO5), unlike that of angiosperms, back-converts thermospermine to norspermidine

FEBS Lett. 2015 Oct 7;589(20 Pt B):3071-8. doi: 10.1016/j.febslet.2015.08.045. Epub 2015 Sep 5.

Authors

G H M Sagor¹, Masataka Inoue¹, Dong Wook Kim¹, Seiji Kojima², Masaru Niitsu³, Thomas Berberich⁴, Tomonobu Kusano⁵

Affiliations

¹ Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan.
² Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan; Frontier Research Institute for Interdisciplinary Sciences, Tohoku University, Aramaki aza Aoba 6-3, Aoba, Sendai 980-8578, Japan.
³ Faculty of Pharmaceutical Sciences, Josai University, Sakado, Saitama 350-0295, Japan.
⁴ Biodiversity and Climate Research Center, Laboratory Center, Georg-Voigt-Str. 14-16, D-60325 Frankfurt am Main, Germany.
⁵ Graduate School of Life Sciences, Tohoku University, 2-1-1 Katahira, Aoba, Sendai 980-8577, Japan. Electronic address: kusano@ige.tohoku.ac.jp.

PMID: 26348400
DOI: 10.1016/j.febslet.2015.08.045

Abstract

In the phylogeny of plant polyamine oxidases (PAOs), clade III members from angiosperms, such as Arabidopsis thaliana PAO5 and Oryza sativa PAO1, prefer spermine and thermospermine as substrates and back-convert both of these substrates to spermidine in vitro. A clade III representative of lycophytes, SelPAO5 from Selaginella lepidophylla, also prefers spermine and thermospermine but instead back-converts these substrates to spermidine and norspermidine, respectively. This finding indicates that the clade III PAOs of lycophytes and angiosperms oxidize thermospermine at different carbon positions. We discuss the physiological significance of this difference.

Keywords: Norspermidine; Polyamine oxidase; Selaginella lepidophylla; Spermidine; Spermine; Thermospermine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Dehydration
Gene Expression Regulation, Plant / drug effects
Molecular Structure
Oxidation-Reduction
Oxidoreductases Acting on CH-NH Group Donors / classification
Oxidoreductases Acting on CH-NH Group Donors / genetics
Oxidoreductases Acting on CH-NH Group Donors / metabolism*
Phylogeny
Plant Proteins / classification
Plant Proteins / genetics
Plant Proteins / metabolism*
Polyamine Oxidase
Reverse Transcriptase Polymerase Chain Reaction
Selaginellaceae / enzymology*
Selaginellaceae / genetics
Selaginellaceae / metabolism
Spectrophotometry
Spermidine / analogs & derivatives*
Spermidine / chemistry
Spermidine / metabolism
Spermine / analogs & derivatives*
Spermine / chemistry
Spermine / metabolism
Tandem Mass Spectrometry
Water / metabolism
Water / pharmacology

Substances

Plant Proteins
Water
Spermine
norspermidine
thermospermine
Oxidoreductases Acting on CH-NH Group Donors
Spermidine