Structure of the polysaccharides from the lipopolysaccharide of Azospirillum brasilense Jm125A2

Elena N Sigida; Yuliya P Fedonenko; Alexander S Shashkov; Evelina L Zdorovenko; Svetlana A Konnova; Vladimir V Ignatov; Yuriy A Knirel

doi:10.1016/j.carres.2015.08.011

Structure of the polysaccharides from the lipopolysaccharide of Azospirillum brasilense Jm125A2

Carbohydr Res. 2015 Oct 30:416:37-40. doi: 10.1016/j.carres.2015.08.011. Epub 2015 Aug 28.

Authors

Elena N Sigida¹, Yuliya P Fedonenko², Alexander S Shashkov³, Evelina L Zdorovenko³, Svetlana A Konnova⁴, Vladimir V Ignatov², Yuriy A Knirel³

Affiliations

¹ Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia. Electronic address: si_elena@mail.ru.
² Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Prospekt Entuziastov 13, 410049 Saratov, Russia.
³ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991 Moscow, Russia.
⁴ Chernyshevsky Saratov State University, Ulitsa Astrakhanskaya 83, 410012 Saratov, Russia.

PMID: 26343325
DOI: 10.1016/j.carres.2015.08.011

Abstract

Two polysaccharides were obtained by mild acid degradation of the lipopolysaccharide of associative nitrogen-fixing bacteria Azospirillum brasilense Jm125A2 isolated from the rhizosphere of a pearl millet. The following structures of the polysaccharides were established by sugar and methylation analyses, Smith degradation, and (1)H and (13)C NMR spectroscopy: [Formula: see text] Structure 1 has been reported earlier for a polysaccharide from A. brasilense S17 (Fedonenko YP, Konnova ON, Zdorovenko EL, Konnova SA, Zatonsky GV, Shaskov AS, Ignatov VV, Knirel YA. Carbohydr Res 2008;343:810-6), whereas to our knowledge structure 2 has not been hitherto found in bacterial polysaccharides.

Keywords: Azospirillum brasilense; Bacterial polysaccharide structure; Lipopolysaccharide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Azospirillum brasilense / chemistry*
Carbohydrate Sequence
Molecular Sequence Data
O Antigens / chemistry*

Substances

O Antigens