More than 30 allergens have been identified from Dermatophagoides farina (D. farina), which is one of the main species of house dust mites. The mite allergens are an important factor contributing to allergic disease in the world. As the detection and identification of new allergens is critical for the diagnosis or treatment of allergic diseases, we sought to characterize the profilin of D. farina (Der f 29) in this study. The results showed that 21% of allergic patients displayed positive results in skin prick test with recombinant Der f 29 (rDer f 29) as the specific allergen; specific IgE reactivity to rDer f 29 was shown by Western Blot and ELISA. In addition, rDer f 29 induced bone marrow-derived dendritic cells (DC) to produce T cells immunoglobulin domain and mucin domain protein 4 (TIM4). Moreover, an allergic asthma mouse model was established by challenging with rDer f 29. Airway hyperresponsiveness, serum specific IgE, IgG1, eosinophil infiltration in the allergic mice bronchoalveolar lavage fluid, the cytokines interleukin-4 (IL-4) and interferon-γ (INF-γ) from spleen cells were markedly increased; the histology showed severe inflammation in the lung. In conclusion, Der f 29 is identified as a new type of the house dust mite allergen.
Keywords: Profilin; dendritic cell; der f 29; expression; purification.