mRNA-Producing Pseudo-nucleus System

Seung Won Shin; Kyung Soo Park; Woo Jung Shin; Soong Ho Um

doi:10.1002/smll.201501334

mRNA-Producing Pseudo-nucleus System

Small. 2015 Nov 4;11(41):5515-9. doi: 10.1002/smll.201501334. Epub 2015 Aug 27.

Authors

Seung Won Shin¹, Kyung Soo Park¹, Woo Jung Shin¹, Soong Ho Um^{1

2}

Affiliations

¹ School of Chemical Engineering, Sungkyunkwan University, Suwon, 440-746, Republic of Korea.
² SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University, Suwon, 440-746, Republic of Korea.

PMID: 26310990
DOI: 10.1002/smll.201501334

Abstract

A pseudo-eukaryotic nucleus (PEN) system consisting of a gene-containing DNA hydrogel encapsulated in a liposome is fabricated. Owing to the structural characteristics of gene-containing DNA hydrogel, mRNA transcription efficiency is promoted 2.57-fold. Through the use of PEN as a platform for mRNA delivery to the cytosol, prolonged protein translation is achieved.

Keywords: DNA nanotechnology; artificial cell nucleus; biomimicry; messenger RNA expression; nucleus mimicry.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Artificial Cells / chemistry*
Cell Nucleus / chemistry*
DNA Ligases / chemistry*
Liposomes / chemistry*
MicroRNAs / chemical synthesis*
Plasmids / chemistry*

Substances

Liposomes
MicroRNAs
DNA Ligases