ROS production in homogenate from the body wall of sea cucumber Stichopus japonicus under UVA irradiation: ESR spin-trapping study

Food Chem. 2016 Feb 1:192:358-62. doi: 10.1016/j.foodchem.2015.07.030. Epub 2015 Jul 8.

Abstract

Sea cucumber Stichopus japonicus (S. japonicus) shows a strong ability of autolysis, which leads to severe deterioration in sea cucumber quality during processing and storage. In this study, to further characterize the mechanism of sea cucumber autolysis, hydroxyl radical production induced by ultraviolet A (UVA) irradiation was investigated. Homogenate from the body wall of S. japonicas was prepared and subjected to UVA irradiation at room temperature. Electron Spin Resonance (ESR) spectra of the treated samples were subsequently recorded. The results showed that hydroxyl radicals (OH) became more abundant while the time of UVA treatment and the homogenate concentration were increased. Addition of superoxide dismutase (SOD), catalase, EDTA, desferal, NaN3 and D2O to the homogenate samples led to different degrees of inhibition on OH production. Metal cations and pH also showed different effects on OH production. These results indicated that OH was produced in the homogenate with a possible pathway as follows: O2(-) → H2O2 → OH, suggesting that OH might be a critical factor in UVA-induced S. japonicus autolysis.

Keywords: DMPO; Homogenate; Hydroxyl radicals; Sea cucumber Stichopus japonicus; UVA irradiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Catalase / metabolism
  • Electron Spin Resonance Spectroscopy
  • Food Analysis / methods*
  • Hydroxyl Radical / analysis
  • Hydroxyl Radical / metabolism
  • Reactive Oxygen Species / analysis*
  • Reactive Oxygen Species / metabolism
  • Stichopus / chemistry*
  • Stichopus / metabolism
  • Stichopus / radiation effects
  • Superoxide Dismutase / metabolism
  • Ultraviolet Rays*

Substances

  • Reactive Oxygen Species
  • Hydroxyl Radical
  • Catalase
  • Superoxide Dismutase