Biotransformation of anthocyanins from two purple-fleshed sweet potato accessions in a dynamic gastrointestinal system

Stan Kubow; Michèle M Iskandar; Kebba Sabally; Behnam Azadi; Shima Sadeghi Ekbatan; Premkumari Kumarathasan; Dharani Dhar Das; Satya Prakash; Gabriela Burgos; Thomas Zum Felde

doi:10.1016/j.foodchem.2015.06.105

Biotransformation of anthocyanins from two purple-fleshed sweet potato accessions in a dynamic gastrointestinal system

Food Chem. 2016 Feb 1:192:171-7. doi: 10.1016/j.foodchem.2015.06.105. Epub 2015 Jun 30.

Authors

Affiliations

¹ School of Dietetics and Human Nutrition, McGill University, 21111 Lakeshore, Ste. Anne de Bellevue, QC H9X 3V9, Canada. Electronic address: stan.kubow@mcgill.ca.
² School of Dietetics and Human Nutrition, McGill University, 21111 Lakeshore, Ste. Anne de Bellevue, QC H9X 3V9, Canada. Electronic address: michele.iskandar@mail.mcgill.ca.
³ School of Dietetics and Human Nutrition, McGill University, 21111 Lakeshore, Ste. Anne de Bellevue, QC H9X 3V9, Canada. Electronic address: kebba.sabally@mcgill.ca.
⁴ School of Dietetics and Human Nutrition, McGill University, 21111 Lakeshore, Ste. Anne de Bellevue, QC H9X 3V9, Canada. Electronic address: behnam.azadi@mcgill.ca.
⁵ School of Dietetics and Human Nutrition, McGill University, 21111 Lakeshore, Ste. Anne de Bellevue, QC H9X 3V9, Canada. Electronic address: shima.sadeghi@mail.mcgill.ca.
⁶ Analytical Biochemistry and Proteomics Laboratory, Mechanistic Studies Division, Healthy Environments and Consumer Safety Branch, Health Canada, 50 Colombine Drwy, Ottawa, ON K1A 0K9, Canada. Electronic address: premkumari.kumarathasan@hc-sc.gc.ca.
⁷ Analytical Biochemistry and Proteomics Laboratory, Mechanistic Studies Division, Healthy Environments and Consumer Safety Branch, Health Canada, 50 Colombine Drwy, Ottawa, ON K1A 0K9, Canada. Electronic address: dharani.das@hc-sc.gc.ca.
⁸ BioMedical Engineering Department, McGill University, 3775 University Street, Room 311, Montreal, QC H3A 2B4, Canada. Electronic address: satya.prakash@mcgill.ca.
⁹ International Potato Center (CIP), Avenida La Molina 1895, La Molina, Apartado Postal 1558 Lima, Peru. Electronic address: g.burgos@cgiar.org.
¹⁰ International Potato Center (CIP), Avenida La Molina 1895, La Molina, Apartado Postal 1558 Lima, Peru. Electronic address: t.zumfelde@cgiar.org.

PMID: 26304335
DOI: 10.1016/j.foodchem.2015.06.105

Abstract

Cooked, milled purple-fleshed sweet potato (PFSP) accessions, PM09.812 and PM09.960, underwent digestion in a dynamic human gastrointestinal (GI) model that simulates gut digestive conditions to study the bioaccessibility and biotransformation of anthocyanins. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry showed accession-dependent variations in anthocyanin release and degradation. After 24h, more anthocyanin species were detected in the small intestinal vessel relative to other vessels for accession PM09.960 whereas more species appeared in the ascending colonic vessel for accession PM09.812. The ferric reducing antioxidant power was increased in the small intestinal vessel for PM09.960 and in the ascending colonic vessel for accession PM09.812, corresponding to the appearance of a majority of anthocyanins for each accession. These results show that intestinal and colonic microbial digestion of PFSP leads to an accession-dependent pattern for anthocyanin bioaccessibility and degradation.

Keywords: Anthocyanins; Bioaccessibility; Biotransformation; Human gastro-intestinal model; Ipomoea batatas L.; Purple fleshed sweet potato.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anthocyanins / analysis
Anthocyanins / metabolism*
Antioxidants / analysis
Antioxidants / metabolism*
Biotransformation
Cooking
Digestion / physiology*
Gastrointestinal Tract / microbiology*
Gastrointestinal Tract / physiology
Humans
Ipomoea batatas / chemistry*
Ipomoea batatas / metabolism
Models, Biological*

Substances

Anthocyanins
Antioxidants