Rapid assay of A2058T-mutated 23S rRNA allelic profiles associated with high-level macrolide resistance in Moraxella catarrhalis

Ryoichi Saito; Ayako Kasai; Shinji Ogihara; Kageto Yamada; Kazuyuki Tao

doi:10.1099/jmm.0.000130

Rapid assay of A2058T-mutated 23S rRNA allelic profiles associated with high-level macrolide resistance in Moraxella catarrhalis

J Med Microbiol. 2015 Sep;64(9):990-992. doi: 10.1099/jmm.0.000130. Epub 2015 Jul 14.

Authors

Ryoichi Saito¹, Ayako Kasai¹, Shinji Ogihara², Kageto Yamada³, Kazuyuki Tao⁴

Affiliations

¹ Department of Microbiology and Immunology, Graduate School of Health Care Sciences, Tokyo Medical and Dental University, Tokyo, Japan.
² Department of Clinical Laboratory, Medical Hospital of Tokyo Medical and Dental University, Tokyo, Japan.
³ Department of Clinical Laboratory, Tokyo Metropolitan Health and Medical Corporation Toshima Hospital, Tokyo, Japan.
⁴ Radioisotope Center, University of Tokyo, Tokyo, Japan.

PMID: 26296581
DOI: 10.1099/jmm.0.000130

Abstract

We report on a restriction fragment-length polymorphism (HpyCH4III) assay for profile analysis of 23S rRNA gene A2058T-mutated alleles associated with high-level macrolide resistance in Moraxella catarrhalis. Our assay results were supported by DNA sequencing analysis, allowed for simultaneous testing of many strains, and produced results from pure-cultured colonies within 4 h.

MeSH terms

Anti-Bacterial Agents / classification
Anti-Bacterial Agents / pharmacology*
DNA, Bacterial / genetics
Drug Resistance, Multiple, Bacterial / genetics*
Macrolides / pharmacology*
Moraxella catarrhalis / drug effects*
Moraxella catarrhalis / genetics
Mutation
Nucleic Acid Amplification Techniques
Polymorphism, Restriction Fragment Length
RNA, Bacterial / genetics*
RNA, Ribosomal, 23S / genetics*

Substances

Anti-Bacterial Agents
DNA, Bacterial
Macrolides
RNA, Bacterial
RNA, Ribosomal, 23S