Engineering of Self-Assembled Fibronectin Matrix Protein and Its Effects on Mesenchymal Stem Cells

Int J Mol Sci. 2015 Aug 19;16(8):19645-56. doi: 10.3390/ijms160819645.

Abstract

Fibronectin (FN) contributes to cell adhesion, proliferation, and differentiation in various cell types. To enhance the activity of fibronectin at the sites of focal adhesion, we engineered a novel recombinant fibronectin (FNIII10) fragment connected to the peptide amphiphile sequence (PA), LLLLLLCCCGGDS. In this study, the effects of FNIII10-PA on rat mesenchymal stem cells (rMSCs) were compared with those of FNIII10. FNIII10-PA showed the prominent protein adhesion activity. In addition, FNIII10-PA showed a significantly higher effect on adhesion, proliferation, and differentiation of rMSCs than FNIII10. Taken together, the FNIII10-containing self-assembled sequence enhanced rMSCs adhesion, proliferation, and differentiation.

Keywords: cellular activity; fibronectin; peptide amphiphile; rat mesenchymal stem cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Adhesion / drug effects
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Fibronectins / chemistry
  • Fibronectins / genetics
  • Fibronectins / pharmacology*
  • Male
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / drug effects*
  • Peptide Fragments / chemistry
  • Peptide Fragments / genetics
  • Protein Engineering / methods*
  • Rats
  • Rats, Sprague-Dawley
  • Recombinant Fusion Proteins / pharmacology

Substances

  • Fibronectins
  • Peptide Fragments
  • Recombinant Fusion Proteins