Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens

S W Peterson; I Martin; W Demczuk; A Bharat; L Hoang; J Wylie; V Allen; B Lefebvre; G Tyrrell; G Horsman; D Haldane; R Garceau; T Wong; M R Mulvey

doi:10.1128/JCM.01632-15

Molecular Assay for Detection of Ciprofloxacin Resistance in Neisseria gonorrhoeae Isolates from Cultures and Clinical Nucleic Acid Amplification Test Specimens

J Clin Microbiol. 2015 Nov;53(11):3606-8. doi: 10.1128/JCM.01632-15. Epub 2015 Aug 19.

Authors

S W Peterson¹, I Martin¹, W Demczuk¹, A Bharat¹, L Hoang², J Wylie³, V Allen⁴, B Lefebvre⁵, G Tyrrell⁶, G Horsman⁷, D Haldane⁸, R Garceau⁹, T Wong¹⁰, M R Mulvey¹¹

Affiliations

¹ Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada.
² British Columbia Centres for Disease Control Public Health Microbiology & Reference Laboratory, Vancouver, British Columbia, Canada.
³ Cadham Provincial Laboratory, Winnipeg, Manitoba, Canada.
⁴ Public Health Ontario Laboratories, Toronto, Ontario, Canada.
⁵ Laboratoire de Santé Publique du Québec, Sainte-Anne-de-Bellevue, Québec, Canada.
⁶ Provincial Laboratory for Public Health, Edmonton, Alberta, Canada.
⁷ Saskatchewan Disease Control Laboratory, Regina, Saskatchewan, Canada.
⁸ Queen Elizabeth II Health Sciences Centre, Halifax, Nova Scotia, Canada.
⁹ Dr. G. L. Dumont Hospital, Moncton, New Brunswick, Canada.
¹⁰ Centre for Communicable Diseases and Infection Control, Public Health Agency of Canada, Ottawa, Ontario, Canada.
¹¹ Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, Manitoba, Canada Department of Medical Microbiology, University of Manitoba, Winnipeg, Manitoba, Canada michael.mulvey@phac-aspc.gc.ca.

Abstract

We developed a real-time PCR assay to detect single nucleotide polymorphisms associated with ciprofloxacin resistance in specimens submitted for nucleic acid amplification testing (NAAT). All three single nucleotide polymorphism (SNP) targets produced high sensitivity and specificity values. The presence of ≥2 SNPs was sufficient to predict ciprofloxacin resistance in an organism.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Canada
Ciprofloxacin / pharmacology*
Cross Reactions
DNA Gyrase / genetics
DNA Topoisomerase IV / genetics
Drug Resistance, Bacterial / genetics*
Gonorrhea / diagnosis
Gonorrhea / microbiology
Humans
Microbial Sensitivity Tests
Neisseria gonorrhoeae / drug effects*
Neisseria gonorrhoeae / genetics*
Neisseria gonorrhoeae / isolation & purification
Nucleic Acid Amplification Techniques / methods*
Polymerase Chain Reaction / methods
Polymorphism, Single Nucleotide / genetics
Sensitivity and Specificity

Substances

Anti-Bacterial Agents
Ciprofloxacin
DNA Topoisomerase IV
DNA Gyrase