1H, 13C and 15N resonance assignments of a C-terminal domain of human CHD1

Biswaranjan Mohanty; Ana P G Silva; Joel P Mackay; Daniel P Ryan

doi:10.1007/s12104-015-9631-1

1H, 13C and 15N resonance assignments of a C-terminal domain of human CHD1

Biomol NMR Assign. 2016 Apr;10(1):31-4. doi: 10.1007/s12104-015-9631-1. Epub 2015 Aug 19.

Authors

Biswaranjan Mohanty^{1

2}, Ana P G Silva¹, Joel P Mackay³, Daniel P Ryan⁴

Affiliations

¹ School of Molecular Bioscience, The University of Sydney, Building G08, Corner Butlin Avenue and Maze Crescent, Sydney, NSW, 2006, Australia.
² Faculty of Pharmacy and Pharmaceutical Sciences, Medicinal Chemistry, Monash Institute of Pharmaceutical Sciences, Monash University, 381 Royal Parade, Parkville, Melbourne, 3052, Australia.
³ School of Molecular Bioscience, The University of Sydney, Building G08, Corner Butlin Avenue and Maze Crescent, Sydney, NSW, 2006, Australia. joel.mackay@sydney.edu.au.
⁴ Department of Genome Sciences, The John Curtin School of Medical Research, The Australian National University, Building 131, Garran Road, Canberra, ACT, 2601, Australia. daniel.ryan@anu.edu.au.

PMID: 26286320
DOI: 10.1007/s12104-015-9631-1

Abstract

Chromatin remodelling proteins are an essential family of eukaryotic proteins. They harness the energy from ATP hydrolysis and apply it to alter chromatin structure in order to regulate all aspects of genome biology. Chromodomain helicase DNA-binding protein 1 (CHD1) is one such remodelling protein that has specialised nucleosome organising abilities and is conserved across eukaryotes. CHD1 possesses a pair of tandem chromodomains that directly precede the core catalytic Snf2 helicase-like domain, and a C-terminal SANT-SLIDE DNA-binding domain. We have identified an additional conserved domain in the C-terminal region of CHD1. Here, we report the backbone and side chain resonance assignments for this domain from human CHD1 at pH 6.5 and 25 °C (BMRB No. 25638).

Keywords: C-terminal domain; CHD1; Chromatin remodelling; Nucleosomes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbon Isotopes
DNA Helicases / chemistry*
DNA-Binding Proteins / chemistry*
Humans
Nitrogen Isotopes
Nuclear Magnetic Resonance, Biomolecular*
Protein Domains
Protein Structure, Secondary
Tritium

Substances

Carbon Isotopes
DNA-Binding Proteins
Nitrogen Isotopes
Tritium
DNA Helicases
CHD1 protein, human