Objective: To investigate the relationship between vitamin D receptor (VDR) gene polymorphisms and chronic periodontitis(CP).
Methods: Buccal swabs from 105 patients with mild/mode-rate CP and 85 severe CP were collected, DNA was extracted from these buccal swabs using the TIANamp Swab DNA Kit [TIANGEN Biotech (Beijing) CO.Ltd]. The VDR rs1544410 and rs731236 were genotyped by the Sequenom MassARRAY system (Shanghai Benegene Biotechnology Co. Ltd), which was based on MALDI-TOF (matrix-assisted laser desorption ionization time-of-flight) technology. The distribution of the genotypes and allele frequencies were analyzed.
Results: The frequencies of the rs1544410 A allele and AA+AG genotype were significantly higher in severe CP than in mild/moderate CP of all the patients and the female patients respectively (all the patients: P=0.006, 0.007; the female patients: P=0.001, 0.001). The frequencies of the rs731236 C allele and CC+CT genotype were significantly higher in severe CP than in mild/moderate CP of all the patients and the female patients respectively (all the patients: P=0.003, 0.004; the female patients: P<0.001, <0.001).
Conclusion: Gene polymorphisms of VDR rs731236 and rs1544410 may be associated with severe CP in Chinese Han population.