Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses

Giulia Cacciotti; Beniamino Caputo; Carla Selvaggi; Andrea la Sala; Laura Vitiello; Diawo Diallo; Cornelia Ceianu; Guido Antonelli; Norbert Nowotny; Carolina Scagnolari

doi:10.1016/j.virol.2015.07.015

Variation in interferon sensitivity and induction between Usutu and West Nile (lineages 1 and 2) viruses

Virology. 2015 Nov:485:189-98. doi: 10.1016/j.virol.2015.07.015. Epub 2015 Aug 14.

Authors

Affiliations

¹ Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185 Rome, Italy.
² Parasitology Unit, Department of Public Health and Infectious Diseases, 'Sapienza' University of Rome, Piazzale Aldo Moro 5, 00185 Rome, Italy.
³ Laboratory of Molecular and Cellular Immunology, IRCCS San Raffaele Pisana, Rome, Italy.
⁴ Unité d'entomologie médicale, Institut Pasteur de Dakar, Senegal.
⁵ Vector-Borne Infections and Medical Entomology Laboratory, "Cantacuzino" National Institute of Research-Development for Microbiology and Immunology, 103 Splaiul Independenţei, 050096 Bucharest, Romania.
⁶ Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, University of Veterinary Medicine Vienna, Veterinärplatz 1, A-1210 Vienna, Austria; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, P.O. Box 35, Al-Khoud, Muscat 123, Oman.
⁷ Department of Molecular Medicine, Laboratory of Virology, 'Sapienza' University of Rome, Viale di Porta Tiburtina 28, 00185 Rome, Italy. Electronic address: carolina.scagnolari@uniroma1.it.

PMID: 26280469
DOI: 10.1016/j.virol.2015.07.015

Abstract

Given the pivotal role of monocyte-derived dendritic cells (DCs) in determining the magnitude of the antiviral innate immune response, we sought to determine whether Usutu virus (USUV) and West Nile virus (WNV) lineages (L)1 and L2 can infect DCs and affect the rate of type I interferon (IFN) activation. The sensitivity of these viruses to types I and III IFNs was also compared. We found that USUV can infect DCs, induce higher antiviral activities, IFN alpha subtypes and the IFN stimulated gene (ISG)15 pathway, and is more sensitive to types I and III IFNs than WNVs. In contrast, we confirmed that IFN alpha/beta subtypes were more effective against WNV L2 than WNV L1. However, the replication kinetics, induction of IFN alpha subtypes and ISGs in DCs and the sensitivity to IFN lambda 1-3 did not differ between WNV L1 and L2.

Keywords: IFN; Interferon; Monocyte-derived dendritic cells; USUV; Usutu virus; WNV; West Nile virus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adaptor Proteins, Signal Transducing
Cell Differentiation
Cytokines / genetics
Cytokines / immunology
Dendritic Cells / drug effects*
Dendritic Cells / immunology
Dendritic Cells / virology
Encephalitis Viruses, Japanese / drug effects*
Encephalitis Viruses, Japanese / genetics
Encephalitis Viruses, Japanese / immunology
Gene Expression Regulation
Host-Pathogen Interactions
Humans
Interferon-alpha / classification
Interferon-alpha / genetics
Interferon-alpha / immunology
Interferon-alpha / pharmacology*
Interferon-beta / classification
Interferon-beta / genetics
Interferon-beta / immunology
Interferon-beta / pharmacology*
Intracellular Signaling Peptides and Proteins / genetics
Intracellular Signaling Peptides and Proteins / immunology
Lipopolysaccharides / pharmacology
Monocytes / cytology
Primary Cell Culture
RNA-Binding Proteins
Signal Transduction
Species Specificity
Transcription Factors / genetics
Transcription Factors / immunology
Ubiquitins / genetics
Ubiquitins / immunology
Viral Load / drug effects
Viral Load / immunology
Virus Replication / drug effects
Virus Replication / immunology
West Nile virus / drug effects*
West Nile virus / genetics
West Nile virus / immunology

Substances

Adaptor Proteins, Signal Transducing
Cytokines
HERC5 protein, human
IFIT1 protein, human
Interferon-alpha
Intracellular Signaling Peptides and Proteins
Lipopolysaccharides
RNA-Binding Proteins
Transcription Factors
Ubiquitins
ISG15 protein, human
Interferon-beta