Abstract
Current methods for detecting disseminated tumor cells in the skeleton are limited by expense and technical complexity. We describe a simple and inexpensive method to quantify, with single cell sensitivity, human metastatic cancer in the mouse skeleton, concurrently with host gene expression, using TRIzol-based DNA/RNA extraction and Alu sequence qPCR amplification. This approach enables precise quantification of tumor cells and corresponding host gene expression during metastatic colonization in xenograft models.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alu Elements / genetics
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Animals
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Biomarkers, Tumor / genetics*
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Bone Neoplasms / genetics*
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Bone Neoplasms / pathology
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Bone Neoplasms / secondary*
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Cell Line, Tumor
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DNA, Neoplasm / genetics
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DNA, Neoplasm / isolation & purification
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Gene Expression Profiling / methods*
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Genetic Markers / genetics
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Guanidines / chemistry
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Mice
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Mice, Nude
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Phenols / chemistry
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RNA, Neoplasm / genetics
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RNA, Neoplasm / isolation & purification
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Real-Time Polymerase Chain Reaction / methods*
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Reproducibility of Results
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Sensitivity and Specificity
Substances
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Biomarkers, Tumor
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DNA, Neoplasm
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Genetic Markers
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Guanidines
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Phenols
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RNA, Neoplasm
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trizol