Seroepidemiology of hepatitis B virus infection in 2 million men aged 21-49 years in rural China: a population-based, cross-sectional study

Jue Liu; Shikun Zhang; Qiaomei Wang; Haiping Shen; Man Zhang; Yiping Zhang; Donghai Yan; Min Liu

doi:10.1016/S1473-3099(15)00218-2

Seroepidemiology of hepatitis B virus infection in 2 million men aged 21-49 years in rural China: a population-based, cross-sectional study

Lancet Infect Dis. 2016 Jan;16(1):80-86. doi: 10.1016/S1473-3099(15)00218-2. Epub 2015 Aug 9.

Authors

Jue Liu¹, Shikun Zhang², Qiaomei Wang², Haiping Shen², Man Zhang³, Yiping Zhang², Donghai Yan², Min Liu⁴

Affiliations

¹ Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, China.
² Department of Maternal and Child Health, National Health and Family Planning Commission of the PRC, Beijing, China.
³ Center for Clinical Laboratory, Beijing Shijitan Hospital, Capital Medical University, Beijing, China.
⁴ Department of Epidemiology and Biostatistics, School of Public Health, Peking University, Beijing, China. Electronic address: liumin@bjmu.edu.cn.

PMID: 26268687
DOI: 10.1016/S1473-3099(15)00218-2

Abstract

Background: Hepatitis B virus (HBV) infection is highly endemic (7-8% prevalence) in rural China, causing high mortality and societal burden. Data from men of reproductive age is scarce and last reported in 2006. We assessed the seroepidemiology of men in rural China, aiming to provide updated baseline data for the prevalence of HBV infection.

Methods: We established prevalence of HBV infection from data gathered through a nationwide population-based study of Chinese rural men aged 21-49 years. Data were obtained from a physical check-up programme for couples of reproductive age, the National Free Preconception Health Examination Project, that covered 31 provinces from 2010-12. We tested serological samples with ELISA and categorised participants' HBV status based on presence of HBsAg, anti-HBV core antibody (anti-HBc), and anti-HBV surface antibody (anti-HBs).

Findings: 2 030 083 men were recruited into the database from Jan 1, 2010, to Dec 31, 2012, and 1 966 013 men provided serum samples for analysis. 124 274 men (6%) tested positive for HBsAg, 178 559 (9%) tested positive for anti-HBc, and 583 923 (30%) tested positive for anti-HBs. Isolated anti-HBs (an indicator of vaccine-mediated immunity) were present in 527 566 men (27%). And 1 234 127 men (63%) were negative for all HBV makers (susceptible population). HBsAg prevalence was higher in men aged 25-39 years (6·35-6·47%) than in other age groups (5·54-5·78%; p<0·0001). HBV markers were all more prevalent in the eastern region of China than in the central or western regions (all p<0·0001). 32 326 (26%) of 124 274 HBsAg-positive men tested positive for HBeAg (suggesting high infectiousness); this decreased with increasing age (p<0·0001).

Interpretation: HBV prevalence in men in rural China has changed from highly endemic into intermediate endemic in the past two decades. However, the absolute number of HBV-infected men and the susceptible population is still very large.

Funding: Chinese Association of Maternal and Child Health Studies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
China / epidemiology
Cross-Sectional Studies
Hepatitis B / epidemiology*
Hepatitis B / virology
Hepatitis B Antibodies / blood*
Hepatitis B Core Antigens / immunology
Hepatitis B Surface Antigens / blood
Hepatitis B Surface Antigens / immunology
Hepatitis B virus / immunology*
Hepatitis B virus / isolation & purification
Humans
Male
Middle Aged
Rural Population
Seroepidemiologic Studies
Young Adult

Substances

Hepatitis B Antibodies
Hepatitis B Core Antigens
Hepatitis B Surface Antigens