Abstract
Arctigenin (ARG) has been previously reported to exert diverse biological activities including anti-proliferation, anti-inflammatory, and antiviral, etc. In the current study, the anti-metastasis and anti-angiogenesis activities of ARG were investigated. To further understand how ARG played these bioactivities, proteomic approaches were used to profile the proteome changes in response to ARG treatment using 2DE-MS/MS. Using these approaches, a total of 50 differentially expressed proteins were identified and clustered. Bioinformatics analysis suggested that multiple signalling pathways were involved. Moreover, ARG induced anti-metastatic and anti-angiogenesis activities were mainly accompanied by a deactivation of the Wnt/β-catenin pathway in HCT116 cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Genetically Modified
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Cell Movement* / drug effects
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Cell Proliferation / drug effects
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Computational Biology
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Computer Simulation*
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Furans / chemistry
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Furans / pharmacology
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Furans / therapeutic use*
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HCT116 Cells
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Histones / metabolism*
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Human Umbilical Vein Endothelial Cells / drug effects
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Human Umbilical Vein Endothelial Cells / metabolism
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Humans
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Lignans / chemistry
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Lignans / pharmacology
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Lignans / therapeutic use*
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Molecular Sequence Data
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Neoplasm Invasiveness
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Neovascularization, Pathologic / drug therapy*
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Protein Interaction Maps / drug effects
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Proteomics
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Wnt Signaling Pathway* / drug effects
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Wound Healing / drug effects
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Zebrafish / genetics
Substances
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Furans
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Histones
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Lignans
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arctigenin