Plasma cytokine levels in dogs undergoing cardiopulmonary bypass

Res Vet Sci. 2015 Aug:101:99-105. doi: 10.1016/j.rvsc.2015.03.011. Epub 2015 May 29.

Abstract

Currently, there are no reports of inflammatory responses to CPB in dogs. We investigated the time course of pro- and anti-inflammatory cytokine levels during and after CPB.

Animals: The study group included 11 dogs that underwent mitral valve repair with CPB, and the control group included 7 healthy dogs that underwent ovariohysterectomy.

Methods: Blood samples from the study group dogs were collected before, during and after surgery and analyzed for plasma levels of interleukin-6 (IL-6), tissue necrosis factor-α (TNF-α), interleukin-10 (IL-10), white blood cells (WBC), and C-reactive protein (CRP). Each inflammatory parameter was also compared with that of the control group dogs.

Results: After CPB, plasma levels of IL-6, WBC counts, and CRP levels were significantly higher than preoperative levels, and IL-6 levels in the study group were significantly higher than those in the control group.

Conclusions: CPB induces a systemic inflammatory response in dogs.

Keywords: C-reactive protein; Cardiopulmonary bypass; Interleukin-10; Interleukin-6; Systemic inflammatory response syndrome; Tissue necrosis factor-α.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • C-Reactive Protein / metabolism
  • Cardiopulmonary Bypass / adverse effects
  • Cardiopulmonary Bypass / veterinary*
  • Cytokines / blood*
  • Dogs
  • Interleukin-10 / blood
  • Interleukin-6 / blood
  • Leukocyte Count / veterinary
  • Mitral Valve / surgery*
  • Systemic Inflammatory Response Syndrome / blood
  • Systemic Inflammatory Response Syndrome / etiology
  • Systemic Inflammatory Response Syndrome / veterinary*
  • Time Factors
  • Tumor Necrosis Factor-alpha / blood

Substances

  • Cytokines
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • C-Reactive Protein