Highly Branched Poly(β-Amino Esters): Synthesis and Application in Gene Delivery

Biomacromolecules. 2015 Sep 14;16(9):2609-17. doi: 10.1021/acs.biomac.5b00966. Epub 2015 Aug 25.

Abstract

Highly branched poly(β-amino esters) (HPAEs) are developed via a facile and controllable "A2+B3/B2" strategy successfully. As nonviral gene delivery vectors, the performance of HPAEs is superior to the well-studied linear counterpart as well as the leading commercial reagent Superfect. When combined with minicircle DNA construct, HPAEs can achieve ultrahigh gene transfection efficiency, especially in keratinocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • DNA / chemistry*
  • Esters* / chemical synthesis
  • Esters* / chemistry
  • Gene Transfer Techniques*
  • Genetic Vectors / chemistry*
  • Keratinocytes / cytology
  • Keratinocytes / metabolism*
  • Mice
  • Mice, Mutant Strains
  • Polyamines* / chemical synthesis
  • Polyamines* / chemistry

Substances

  • Esters
  • Polyamines
  • DNA