Droplet-Array (DA) Sandwich Chip: A Versatile Platform for High-Throughput Cell Screening Based on Superhydrophobic-Superhydrophilic Micropatterning

Anna A Popova; Sebastian M Schillo; Konstantin Demir; Erica Ueda; A Nesterov-Mueller; Pavel A Levkin

doi:10.1002/adma.201502115

Droplet-Array (DA) Sandwich Chip: A Versatile Platform for High-Throughput Cell Screening Based on Superhydrophobic-Superhydrophilic Micropatterning

Adv Mater. 2015 Sep 16;27(35):5217-22. doi: 10.1002/adma.201502115. Epub 2015 Aug 10.

Authors

Anna A Popova¹, Sebastian M Schillo², Konstantin Demir¹, Erica Ueda¹, A Nesterov-Mueller², Pavel A Levkin¹

Affiliations

¹ Karlsruhe Institute of Technology, Institute of Toxicology and Genetics, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.
² Karlsruhe Institute of Technology, Institute of Microstructure Technology, Hermann-von-Helmholtz-Platz 1, 76344, Eggenstein-Leopoldshafen, Germany.

PMID: 26255809
DOI: 10.1002/adma.201502115

Abstract

A droplet-array (DA) sandwich chip is a miniaturized platform for cell-based high-throughput screening. It is based on sandwiching of a glass slide with a preprinted library and a superhydrophobic-superhydrophilic pattern, which consists of thousands of simultaneously formed microdroplets containing cells. The DA sandwich chip allows for one-step cell seeding, simultaneous initiation of screening, and 1000 times less reagent consumption than a regular 96-well plate.

Keywords: droplet-array sandwich chips; high throughput screening; superhydrophobic-superhydrophilic micropatterning.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Survival
HEK293 Cells
HeLa Cells
Humans
Hydrophobic and Hydrophilic Interactions*
Microfluidic Analytical Techniques / instrumentation*
Tissue Array Analysis / instrumentation*