A self-assembled system for tumor-targeted co-delivery of drug and gene

Mater Sci Eng C Mater Biol Appl. 2015 Nov 1:56:280-5. doi: 10.1016/j.msec.2015.06.034. Epub 2015 Jun 20.

Abstract

A new cationic polymer eprosartan-graft-PEI (ESP) containing eprosartan (ES) and polyethylenimine 1.8K was successfully developed and employed as a safe gene vector to assemble a drug (ES) and gene co-delivery complex (ESP/pDNA). Chondroitin sulfate (CS) was then used as a coating polymer to shield the surface charge of ESP/pDNA complexes, as well as a tumor targeting entity to ensure specific delivery of CS/ESP/pDNA complexes. The CS/ESP/pDNA complexes with desirable particle size and zeta potential, exhibited amidase-responsive drug release and CS-mediated endocytosis in vitro. As compared with ESP/pDNA complexes, in vivo imaging results demonstrated decreased reticuloendothelial system uptake and remarkably increased tumor accumulation of CS/ESP/pDNA complexes. All these findings indicated the potential of CS/ESP/pDNA as a promising tumor-targeted drug and gene co-delivery system.

Keywords: Co-delivery; Drug; Gene; Self-assembly; Tumor-targeted.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylates* / chemistry
  • Acrylates* / pharmacokinetics
  • Acrylates* / pharmacology
  • Chondroitin Sulfates* / chemistry
  • Chondroitin Sulfates* / pharmacokinetics
  • Chondroitin Sulfates* / pharmacology
  • Drug Delivery Systems / methods*
  • Endocytosis
  • Gene Transfer Techniques*
  • Hep G2 Cells
  • Humans
  • Imidazoles* / chemistry
  • Imidazoles* / pharmacokinetics
  • Imidazoles* / pharmacology
  • Neoplasms
  • Plasmids* / chemistry
  • Plasmids* / pharmacokinetics
  • Plasmids* / pharmacology
  • Polyethyleneimine* / chemistry
  • Polyethyleneimine* / pharmacokinetics
  • Polyethyleneimine* / pharmacology
  • Thiophenes* / chemistry
  • Thiophenes* / pharmacokinetics
  • Thiophenes* / pharmacology

Substances

  • Acrylates
  • Imidazoles
  • Thiophenes
  • eprosartan
  • Polyethyleneimine
  • Chondroitin Sulfates