Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line with Powdery Mildew Resistance

Diaoguo An; Qi Zheng; Qiaoling Luo; Pengtao Ma; Hongxia Zhang; Lihui Li; Fangpu Han; Hongxing Xu; Yunfeng Xu; Xiaotian Zhang; Yilin Zhou

doi:10.1371/journal.pone.0134534

Molecular Cytogenetic Identification of a New Wheat-Rye 6R Chromosome Disomic Addition Line with Powdery Mildew Resistance

PLoS One. 2015 Aug 3;10(8):e0134534. doi: 10.1371/journal.pone.0134534. eCollection 2015.

Authors

Diaoguo An¹, Qi Zheng², Qiaoling Luo¹, Pengtao Ma¹, Hongxia Zhang¹, Lihui Li³, Fangpu Han², Hongxing Xu¹, Yunfeng Xu¹, Xiaotian Zhang¹, Yilin Zhou⁴

Affiliations

¹ Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China.
² The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
³ The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
⁴ The State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.

Abstract

Rye (Secale cereale L.) possesses many valuable genes that can be used for improving disease resistance, yield and environment adaptation of wheat (Triticum aestivum L.). However, the documented resistance stocks derived from rye is faced severe challenge due to the variation of virulent isolates in the pathogen populations. Therefore, it is necessary to develop desirable germplasm and search for novel resistance gene sources against constantly accumulated variation of the virulent isolates. In the present study, a new wheat-rye line designated as WR49-1 was produced through distant hybridization and chromosome engineering protocols between common wheat cultivar Xiaoyan 6 and rye cultivar German White. Using sequential GISH (genomic in situ hybridization), mc-FISH (multicolor fluorescence in situ hybridization), mc-GISH (multicolor GISH) and EST (expressed sequence tag)-based marker analysis, WR49-1 was proved to be a new wheat-rye 6R disomic addition line. As expected, WR49-1 showed high levels of resistance to wheat powdery mildew (Blumeria graminis f. sp. tritici, Bgt) pathogens prevalent in China at the adult growth stage and 19 of 23 Bgt isolates tested at the seedling stage. According to its reaction pattern to different Bgt isolates, WR49-1 may possess new resistance gene(s) for powdery mildew, which differed from the documented powdery mildew gene, including Pm20 on chromosome arm 6RL of rye. Additionally, WR49-1 was cytologically stable, had improved agronomic characteristics and therefore could serve as an important bridge for wheat breeding and chromosome engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ascomycota*
Chromosome Mapping
Cytogenetics
Disease Resistance / genetics*
Genes, Plant
Mycoses / genetics*
Plant Breeding*
Plant Diseases / genetics*
Secale / genetics*
Triticum / genetics*

Grants and funding

This work was supported by the National Natural Science Foundation of China (31171550) (http://www.nsfc.gov.cn/) to DGA, National High-Tech Research and Development Program of China (2011AA1001) (http://www.863.gov.cn/) to DGA, and National Scientific and Technological Supporting Program of China (2013BAD01B02) (http://www.most.gov.cn/) to DGA. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.