High prevalence of fluoroquinolone-nonsusceptible Streptococcus pyogenes emm12 in Taiwan

Jiun-Nong Lin; Lin-Li Chang; Chung-Hsu Lai; Yi-Han Huang; Wei-Fang Chen; Chih-Hui Yang; Janine Hsu; Hsi-Hsun Lin; Yen-Hsu Chen

doi:10.1016/j.diagmicrobio.2015.06.018

High prevalence of fluoroquinolone-nonsusceptible Streptococcus pyogenes emm12 in Taiwan

Diagn Microbiol Infect Dis. 2015 Oct;83(2):187-92. doi: 10.1016/j.diagmicrobio.2015.06.018. Epub 2015 Jun 30.

Authors

Jiun-Nong Lin¹, Lin-Li Chang², Chung-Hsu Lai³, Yi-Han Huang⁴, Wei-Fang Chen³, Chih-Hui Yang⁵, Janine Hsu⁶, Hsi-Hsun Lin³, Yen-Hsu Chen⁷

Affiliations

¹ Department of Critical Care Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan; School of Medicine, College of Medicine, I-Shou University, Kaohsiung, Taiwan; Division of Infectious Diseases, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
² Department of Microbiology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.
³ Division of Infectious Diseases, Department of Internal Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
⁴ Department of Critical Care Medicine, E-Da Hospital, I-Shou University, Kaohsiung, Taiwan.
⁵ General Education Center, Meiho University, Pingtung, Taiwan.
⁶ Department of Microbiology and Immunology, McGill University, Montreal, Canada.
⁷ School of Medicine, Sepsis Research Center, Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan; Division of Infectious Diseases, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan; Department of Biological Science and Technology, College of Biological Science and Technology, National Chiao Tung University, Hsinchu, Taiwan. Electronic address: infchen@gmail.com.

PMID: 26234479
DOI: 10.1016/j.diagmicrobio.2015.06.018

Abstract

Fluoroquinolone-nonsusceptible Streptococcus pyogenes has rapidly emerged in several countries. The aim of this study was to survey the epidemiology and molecular characteristics of fluoroquinolone-nonsusceptible S. pyogenes in Taiwan. A total of 350 consecutive S. pyogenes isolates were collected between January 2005 and December 2012, including 152 (43.4%) invasive and 198 (56.6%) noninvasive isolates. Thirty-nine isolates (11.1%) of S. pyogenes were nonsusceptible to fluoroquinolones, including one emm1/ST28, 4 emm4/ST39, 33 emm12/ST36, and 1 emm87/ST62. Of all the isolates, emm12 (50%) demonstrated the highest prevalence of fluoroquinolone nonsusceptibility. Alterations of Ser79Phe and Ala12Val in ParC were the most frequently mutations in fluoroquinolone-nonsusceptible S. pyogenes isolates. There were no amino acid substitutions in GyrB, and 1 emm87 isolate exhibited 3 nonsynonymous mutations in ParE. Our study reveals the emergence of fluoroquinolone-nonsusceptible S. pyogenes emm12/ST36 in Taiwan. Regular surveillance of fluoroquinolone susceptibility in S. pyogenes is suggested.

Keywords: Fluoroquinolone resistance; Group A streptococcus; Quinolone resistance-determining regions; Streptococcus pyogenes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Anti-Bacterial Agents / pharmacology*
DNA Gyrase / genetics
DNA Topoisomerase IV / genetics
Drug Resistance, Bacterial*
Electrophoresis, Gel, Pulsed-Field
Fluoroquinolones / pharmacology*
Genotype
Humans
Molecular Typing
Mutation, Missense
Prevalence
Streptococcal Infections / epidemiology*
Streptococcal Infections / microbiology*
Streptococcus pyogenes / classification
Streptococcus pyogenes / drug effects*
Streptococcus pyogenes / genetics
Streptococcus pyogenes / isolation & purification*
Taiwan / epidemiology

Substances

Anti-Bacterial Agents
Fluoroquinolones
DNA Topoisomerase IV
DNA Gyrase