Genomic characterization of a novel avian arthritis orthoreovirus variant by next-generation sequencing

Yi Tang; Huaguang Lu

doi:10.1007/s00705-015-2547-3

Genomic characterization of a novel avian arthritis orthoreovirus variant by next-generation sequencing

Arch Virol. 2015 Oct;160(10):2629-32. doi: 10.1007/s00705-015-2547-3. Epub 2015 Aug 4.

Authors

Yi Tang¹, Huaguang Lu²

Affiliations

¹ Wiley Lab/Avian Virology, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, 16802, USA.
² Wiley Lab/Avian Virology, Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, PA, 16802, USA. hxl15@psu.edu.

PMID: 26234183
DOI: 10.1007/s00705-015-2547-3

Abstract

By using next-generation sequencing (NGS) technology, we have identified a divergent avian orthoreovirus (ARV) field variant (Reo/PA/Broiler/15511/13, or PA15511), isolated from broiler chickens with viral arthritis in Pennsylvania in 2013. The complete genome of the PA15511 field strain was 23,495 bp in length with 10 dsRNA segments encoding 12 viral proteins. The lengths of the genomic segments ranged from 1192 bp (S4) to 3958 bp (L1). Genomic analysis has revealed that this virus is distinct from reference ARV strains and meets criteria for a new or novel strain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Chickens
Genome, Viral
Genomics
High-Throughput Nucleotide Sequencing
Molecular Sequence Data
Orthoreovirus, Avian / genetics*
Orthoreovirus, Avian / isolation & purification*
Orthoreovirus, Avian / physiology
Phylogeny
Poultry Diseases / virology*
Reoviridae Infections / veterinary*
Reoviridae Infections / virology