Highly sensitive electrochemical biosensor for bisphenol A detection based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled carbon nanotube-tyrosinase hybrid film

Nedjla Zehani; Philippe Fortgang; Mohamed Saddek Lachgar; Abdoullatif Baraket; Madjid Arab; Sergei V Dzyadevych; Rochdi Kherrat; Nicole Jaffrezic-Renault

doi:10.1016/j.bios.2015.07.051

Highly sensitive electrochemical biosensor for bisphenol A detection based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled carbon nanotube-tyrosinase hybrid film

Biosens Bioelectron. 2015 Dec 15:74:830-5. doi: 10.1016/j.bios.2015.07.051. Epub 2015 Jul 26.

Authors

Nedjla Zehani¹, Philippe Fortgang², Mohamed Saddek Lachgar³, Abdoullatif Baraket², Madjid Arab⁴, Sergei V Dzyadevych⁵, Rochdi Kherrat³, Nicole Jaffrezic-Renault⁶

Affiliations

¹ University of Lyon, Institute of Analytical Sciences, UMR 5280, 5 rue de la Doua, Villeurbanne 69100, France; University of Annaba, Faculty of Engineering Sciences, Laboratory of Environmental Engineering, B.P.12, Annaba 23000, Algeria.
² University of Lyon, Institute of Analytical Sciences, UMR 5280, 5 rue de la Doua, Villeurbanne 69100, France.
³ University of Annaba, Faculty of Engineering Sciences, Laboratory of Environmental Engineering, B.P.12, Annaba 23000, Algeria.
⁴ University of Sud ToulonVar - Institute of Materials Microelectronics and Nanosciences of Provence, UMR CNRS 7334, La Garde, France.
⁵ Laboratory of Biomolecular Electronics, Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, 150 Zabolotnogo Street, Kiev 03143, Ukraine.
⁶ University of Lyon, Institute of Analytical Sciences, UMR 5280, 5 rue de la Doua, Villeurbanne 69100, France. Electronic address: nicole.jaffrezic@univ-lyon1.fr.

PMID: 26232678
DOI: 10.1016/j.bios.2015.07.051

Abstract

A highly sensitive electrochemical biosensor for the detection of Bisphenol A (BPA) in water has been developed by immobilizing tyrosinase onto a diazonium-functionalized boron doped diamond electrode (BDD) modified with multi-walled carbon nanotubes (MWCNTs). The fabricated biosensor exhibits excellent electroactivity towards o-quinone, a product of this enzymatic reaction of BPA oxidation catalyzed by tyrosinase. The developed BPA biosensor displays a large linear range from 0.01 nM to 100 nM, with a detection limit (LOD) of 10 pM. The feasibility of the proposed biosensor has been demonstrated on BPA spiked water river samples. Therefore, it could be a promising and reliable analytical tool for on-site monitoring of BPA in waste water.

Keywords: Biosensor; Bisphenol A; Diamond electrode; Diazonium; Tyrosinase.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Benzhydryl Compounds / chemistry
Benzhydryl Compounds / isolation & purification*
Biosensing Techniques*
Boron / chemistry
Diazonium Compounds
Electrochemistry*
Enzymes, Immobilized / chemistry
Limit of Detection
Monophenol Monooxygenase / chemistry
Nanotubes, Carbon / chemistry*
Phenols / chemistry
Phenols / isolation & purification*
Wastewater / chemistry

Substances

Benzhydryl Compounds
Diazonium Compounds
Enzymes, Immobilized
Nanotubes, Carbon
Phenols
Waste Water
Monophenol Monooxygenase
bisphenol A
Boron