An improved method for HLA-B and -C supratyping

J Immunol Methods. 2015 Nov:426:29-34. doi: 10.1016/j.jim.2015.07.008. Epub 2015 Jul 29.

Abstract

A growing body of evidence links the analysis of the KIR genotype and the presence of their HLA-B and -C ligands to a wide repertoire of human diseases. We noticed that, using a panel of 184 Caucasoid donors, a limited number of HLA alleles were incorrectly supratyped by previously described pyrosequence-based assays. Here we describe a simple implementation of the reported methods that corrects all the discrepancies found with HLA-B and -C molecular typing and allows establishing a quick and high-throughput method for the determination of HLA-Bw4 I(80), Bw4T(80), Bw6 and HLA-C1 or -C2 supratype.

Keywords: Genotyping; HLA; HLA-B (Bw4/Bw6); HLA-C (C1/C2); KIR; NK cells; Pyrosequencing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Base Sequence
  • HLA-B Antigens / classification*
  • HLA-B Antigens / genetics
  • HLA-C Antigens / classification*
  • HLA-C Antigens / genetics
  • High-Throughput Nucleotide Sequencing / methods*
  • Histocompatibility Testing / methods*
  • Humans
  • Killer Cells, Natural / immunology
  • Molecular Typing / methods
  • Receptors, KIR / genetics
  • Sequence Analysis, DNA / methods*
  • T-Lymphocytes / immunology

Substances

  • HLA-B Antigens
  • HLA-Bw4 antigen
  • HLA-Bw6 antigen
  • HLA-C Antigens
  • Receptors, KIR