Mesoporous materials modified by aptamers and hydrophobic groups assist ultra-sensitive insulin detection in serum

Chem Commun (Camb). 2015 Sep 14;51(71):13642-5. doi: 10.1039/c5cc04458h.

Abstract

A novel mesoporous material modified with both insulin-binding-aptamers and hydrophobic methyl groups is synthesized. With rationally designed pore structures and surface chemistry, this material is applied in sample pre-treatment for ELISA, and enables the quantification (0.25-5 pg ml(-1)) of insulin in serum, 30-fold enhancement of the limit-of-detection compared to the commercial ELISA kit.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aptamers, Nucleotide / metabolism*
  • Blood Chemical Analysis / methods*
  • Enzyme-Linked Immunosorbent Assay
  • Horseradish Peroxidase / chemistry
  • Horseradish Peroxidase / metabolism
  • Hydrophobic and Hydrophilic Interactions
  • Insulin / blood*
  • Microscopy, Electron, Transmission
  • Molecular Structure
  • Oligonucleotides / chemistry
  • Oligonucleotides / metabolism
  • Porosity

Substances

  • Aptamers, Nucleotide
  • Insulin
  • Oligonucleotides
  • Horseradish Peroxidase