Abstract
A novel mesoporous material modified with both insulin-binding-aptamers and hydrophobic methyl groups is synthesized. With rationally designed pore structures and surface chemistry, this material is applied in sample pre-treatment for ELISA, and enables the quantification (0.25-5 pg ml(-1)) of insulin in serum, 30-fold enhancement of the limit-of-detection compared to the commercial ELISA kit.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aptamers, Nucleotide / metabolism*
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Blood Chemical Analysis / methods*
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Enzyme-Linked Immunosorbent Assay
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Horseradish Peroxidase / chemistry
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Horseradish Peroxidase / metabolism
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Hydrophobic and Hydrophilic Interactions
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Insulin / blood*
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Microscopy, Electron, Transmission
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Molecular Structure
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Oligonucleotides / chemistry
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Oligonucleotides / metabolism
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Porosity
Substances
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Aptamers, Nucleotide
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Insulin
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Oligonucleotides
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Horseradish Peroxidase