We revise the Afrotropical Lycorininae and describe Lycorina yui Rousse & van Noort sp. nov. from South Africa. An illustrated key to Lycorininae species of the Afrotropical region is provided. Lycorina continentalis (Benoit, 1953) is considered a junior synonym of Lycorinafici Seyrig, 1932, and is newly reported from Uganda and South Africa. Online dichotomous and interactive Lucid keys are available at http://www.waspweb.org.