Differential proteomes of the cyanobacterium Cyanothece sp. CCY 0110 upon exposure to heavy metals

Rita Mota; Sara B Pereira; Marianna Meazzini; Rui Fernandes; Arlete Santos; Caroline A Evans; Roberto De Philippis; Phillip C Wright; Paula Tamagnini

doi:10.1016/j.dib.2015.04.015

Differential proteomes of the cyanobacterium Cyanothece sp. CCY 0110 upon exposure to heavy metals

Data Brief. 2015 May 22:4:152-8. doi: 10.1016/j.dib.2015.04.015. eCollection 2015 Sep.

Authors

Rita Mota¹, Sara B Pereira², Marianna Meazzini³, Rui Fernandes², Arlete Santos¹, Caroline A Evans⁴, Roberto De Philippis⁵, Phillip C Wright⁴, Paula Tamagnini¹

Affiliations

¹ Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal ; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal ; Faculdade de Ciências, Departamento de Biologia, Universidade do Porto, Porto, Portugal.
² Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal ; IBMC - Instituto de Biologia Molecular e Celular, Universidade do Porto, Porto, Portugal.
³ Department of Agrifood Production and Environmental Sciences, University of Florence, Florence, Italy.
⁴ ChELSI Institute, Department of Chemical and Biological Engineering, University of Sheffield, Sheffield, United Kingdom.
⁵ Department of Agrifood Production and Environmental Sciences, University of Florence, Florence, Italy ; Institute of Ecosystem Study (ISE), National Research Council (CNR), Sesto Fiorentino (FI), Italy.

Abstract

The proteomes of the highly efficient extracellular polymeric substances (EPS)-producer cyanobacterium Cyanothece sp. CCY 0110, grown in medium supplemented with an essential metal (Cu(2+)) or a non-essential metal (Cd(2+)),were compared using iTRAQ technology. The data were obtained within a larger study that evaluated the overall effects of different heavy metals on growth/survival, EPS production and ultrastructure of this cyanobacterium [1]. To allow a broader understanding of the strategies triggered to coupe with toxic effects of the metals, Cyanothece's proteomes were evaluated after chronic and acute exposure to Cu(2+) and Cd(2+) in two independent 8-plex iTRAQ studies. For the chronic exposure 0.1 mg/l of Cu(2+) or 5 mg/l of Cd(2+) were used for 10 and 20 days, while in the acute experiments the cells were exposed to 10× these concentrations for 24 h. 202 and 268 proteins were identified and quantified for studies 1 (Cu(2+)) and 2 (Cd(2+)), respectively. The majority of the proteins with significant fold changes were associated with photosynthesis, CO2 fixation and carbohydrate metabolism, translation, and nitrogen and amino acid metabolism.

Keywords: Cadmium; Copper; Cyanobacteria; Cyanothece; Proteome; iTRAQ.