Evaluation of chemical constitute, fatty acids and antioxidant activity of the fruit and seed of sea buckthorn (Hippophae rhamnoides L.) grown wild in Iran

Nat Prod Res. 2016;30(3):366-8. doi: 10.1080/14786419.2015.1057728. Epub 2015 Jul 27.

Abstract

In this investigation, the chemical compositions of berries from sea buckthorn were studied. The amount of ascorbic acid and β-carotene determined by HPLC was 170 mg/100 g FW and 0.20 mg/g FW, respectively. Total phenols, anthocyanins, acidity and total soluble solids (TSS) contents were 247 mg GAE/100 g FW, 3 mg/L (cyanidin-3-glucoside), 5.32% and 13.8%, respectively. Fruit antioxidant activity determined by the ferric reducing ability of plasma (FRAP) method was 24.85 mM Fe/100 g FW. Results confirmed the presence of six dominant fatty acids (determined by GC) in fruit including linoleic (34.2%), palmitoleic (21.37%), palmitic (17.2%), oleic (12.8%), linolenic (5.37%) and stearic acid (1.67%). Five dominant fatty acids of the seeds were linoleic (42.36%), linolenic (21.27%), oleic (21.34%), palmitic (6.54%) and stearic acid (2.54%). The nitrogen content was 3.96%. The P, K, Ca, Mg, Fe, Zn, Mn, Cu, Cd and Cl contents of fruit were 491, 1674, 1290, 990, 291, 29.77, 108.37, 17.87, 0.021 and 2.18 mg/kg DW, respectively.

Keywords: ascorbic acid; fatty acid; fruit; sea buckthorn; seed.

MeSH terms

  • Anthocyanins / analysis
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Chromatography, Gas
  • Fatty Acids / analysis*
  • Fatty Acids / chemistry
  • Fruit / chemistry*
  • Glucosides / analysis
  • Hippophae / chemistry*
  • Iran
  • Metals / analysis
  • Nitrogen / analysis
  • Seeds / chemistry*
  • beta Carotene / analysis

Substances

  • Anthocyanins
  • Antioxidants
  • Fatty Acids
  • Glucosides
  • Metals
  • cyanidin-3-O-beta-glucopyranoside
  • beta Carotene
  • Nitrogen