System for evaluation of oxidative stress on in-vitro-produced bovine embryos

Reprod Biomed Online. 2015 Oct;31(4):577-80. doi: 10.1016/j.rbmo.2015.06.014. Epub 2015 Jul 3.

Abstract

This study proposed a quantitative evaluation of oxidative status (OS) in bovine embryos. Sixteen-cell stage embryos, cultured under 5% O2, were treated with oxidative stress inducer menadione (0, 1, 2.5 and 5 µmol/l) for 24 h. Blastocyst rate (BLR) was recorded and expanded blastocysts were stained with CellROX®Green (CRG; OS evaluation) and evaluated under epifluorescence microscopy (ratio of pixel/blastomere). A significant effect of menadione was observed for BLR (P = 0.0039), number of blastomeres/embryo (P < 0.0001) and OS (P < 0.001). Strong negative correlations were found between BLR and the number of blastomeres with OS evaluation, demonstrating the efficacy of this analysis to evaluate OS levels of IVF bovine embryos.

Keywords: CellROX®Green; development; embryo oxidative stress; microscopy of epifluorescence.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastocyst / cytology
  • Blastocyst / drug effects
  • Blastocyst / metabolism
  • Blastomeres / cytology
  • Blastomeres / drug effects
  • Blastomeres / metabolism
  • Cattle
  • Embryo Culture Techniques
  • Embryo, Mammalian / cytology
  • Embryo, Mammalian / drug effects
  • Embryo, Mammalian / metabolism*
  • Embryonic Development / drug effects
  • Embryonic Development / physiology
  • Female
  • Fertilization in Vitro / veterinary
  • Microscopy, Fluorescence
  • Oxidative Stress* / drug effects
  • Vitamin K 3 / toxicity

Substances

  • Vitamin K 3