Abstract
A copper-(I)-catalyzed variation of the Huisgen 1,3-dipolar cycloaddition has been applied to lead the in living-cell mass-spectrometry based identification of protein targets of oleocanthal, a natural metabolite daily ingested by millions of people. Chemical proteomics revealed heat-shock proteins, HSP70 and HSP90, as main oleocanthal interactors in living systems. These two proteins are involved in cancer development and, thus, our findings could have important outcomes for a deep evaluation of the bio-pharmacological significance of oleocanthal.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Aldehydes / chemistry
-
Aldehydes / metabolism*
-
Cyclopentane Monoterpenes
-
HSP70 Heat-Shock Proteins / chemistry
-
HSP70 Heat-Shock Proteins / metabolism
-
HSP90 Heat-Shock Proteins / chemistry
-
HSP90 Heat-Shock Proteins / metabolism
-
Humans
-
Mass Spectrometry
-
Olive Oil
-
Phenols / chemistry
-
Phenols / metabolism*
-
Plant Oils / chemistry
-
Plant Oils / metabolism*
-
Protein Binding
-
Proteomics
Substances
-
Aldehydes
-
Cyclopentane Monoterpenes
-
HSP70 Heat-Shock Proteins
-
HSP90 Heat-Shock Proteins
-
Olive Oil
-
Phenols
-
Plant Oils
-
oleocanthal