A newly developed transcription-mediated amplification assay was used to detect chikungunya virus infection in 3 of 557 asymptomatic donors (0.54%) from Puerto Rico during the 2014-2015 Caribbean epidemic. Viral detection was confirmed by using PCR, microarray, and next-generation sequencing. Molecular clock analysis dated the emergence of the Puerto Rico strains to early 2013.
Keywords: ViroChip microarray; blood donor; chikungunya virus; next-generation sequencing; transcription-mediated amplification; transfusion-transmitted infection; viruses; whole-genome sequencing.