Metabolomics for characterization of gender differences in patients infected with dengue virus

Nurul Shahfiza; Hasnah Osman; Tang T Hock; Khozirah Shaari; Abdel-Hamid Z Abdel-Hamid

doi:10.1016/j.apjtm.2015.05.012

Metabolomics for characterization of gender differences in patients infected with dengue virus

Asian Pac J Trop Med. 2015 Jun;8(6):451-6. doi: 10.1016/j.apjtm.2015.05.012. Epub 2015 Jun 25.

Authors

Nurul Shahfiza¹, Hasnah Osman², Tang T Hock¹, Khozirah Shaari³, Abdel-Hamid Z Abdel-Hamid⁴

Affiliations

¹ Advanced Medical and Dental Institute, University Science Malaysia, Malaysia.
² School of Chemical Sciences, University Science Malaysia, Malaysia.
³ Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Malaysia.
⁴ Advanced Medical and Dental Institute, University Science Malaysia, Malaysia; National Research Centre, Cairo, Egypt. Electronic address: abdelhamidzaki@hotmail.com.

PMID: 26194829
DOI: 10.1016/j.apjtm.2015.05.012

Abstract

Objective: To determine the metabolic response associate with dengue infection based on human gender metabolic differences by means of (1)H NMR-spectrometry.

Methods: The mid-stream urine collected from both male and female patients diagnosed with dengue fever at Penang General Hospital and fourty-three healthy individuals were analyzed with (1)H NMR spectroscopy, followed by chemometric multivariate analysis. NMR signals which highlighted in the OPLS-DA S-plot were further selected and identified using Human Metabolome Database, Chenomx Profiler.

Results: The results pointed out that NMR urine profiling was able to capture human gender metabolic differences that are important for the distinction of classes of individuals of similar physiological conditions; infected with dengue. Distinct differences between dengue infected patients versus healthy individuals and subtle differences in male versus female infected with dengue were found to be related to the metabolism of amino acid and tricarboxylic acid intermediates cycle.

Conclusions: The (1)H NMR metabolomic investigation combined with appropriate algorithms and pattern recognition procedures, gave an evidence for the existence of distinct metabolic differentiation of individuals, according to their gender, modulates with the infection risk.

Keywords: Dengue infection; Metabolomics; NMR-spectroscopy.