The use of dried blood spot specimens for HIV-1 drug resistance genotyping in young children initiating antiretroviral therapy

Anna T Salimo; Johanna Ledwaba; Ashraf Coovadia; Elaine J Abrams; Karl-Günter Technau; Louise Kuhn; Lynn Morris; Gillian M Hunt

doi:10.1016/j.jviromet.2015.07.005

The use of dried blood spot specimens for HIV-1 drug resistance genotyping in young children initiating antiretroviral therapy

J Virol Methods. 2015 Oct:223:30-2. doi: 10.1016/j.jviromet.2015.07.005. Epub 2015 Jul 17.

Authors

Anna T Salimo¹, Johanna Ledwaba², Ashraf Coovadia³, Elaine J Abrams⁴, Karl-Günter Technau⁵, Louise Kuhn⁶, Lynn Morris⁷, Gillian M Hunt⁸

Affiliations

¹ Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa. Electronic address: Anna.Salimo@nhls.ac.za.
² Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa. Electronic address: Johannal@nicd.ac.za.
³ Empilweni Services and Research Unit, Rahima Moosa Mother and Child Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: ashraf.coovadia@wits.ac.za.
⁴ ICAP, Mailman School of Public Health, and College of Physicians & Surgeons, Columbia University, New York, USA. Electronic address: eja1@cumb.columbia.edu.
⁵ Empilweni Services and Research Unit, Rahima Moosa Mother and Child Hospital, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: karl-gunter.technau@wits.ac.za.
⁶ Gertrude H. Sergievsky Center, College of Physicians and Surgeons, and Department of Epidemiology, Mailman School of Public Health, Columbia University, New York, USA. Electronic address: lk24@cumc.columbia.edu.
⁷ Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: lynnm@nicd.ac.za.
⁸ Centre for HIV and STIs, National Institute for Communicable Diseases, National Health Laboratory Services, Johannesburg, South Africa; Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa. Electronic address: gillianh@nicd.ac.za.

Abstract

Paired plasma and dried blood spots (DBS) from 232 South African HIV-infected children initiating antiretroviral therapy (ART) were genotyped for drug resistance mutations, most of who had prior exposure to ART for prevention-of-mother-to-child-transmission. Non-nucleoside reverse transcriptase inhibitor mutations were most commonly detected in both specimen types, particularly Y181C/I and K103N/S. Resistance interpretation concordance was achieved in 97% of pairs with seven children having mutations detected in DBS only. These results validate the preferential use of DBS specimens for HIVDR genotyping in this patient group.

Keywords: Children; Dried blood spots; HIV drug resistance genotyping.

Publication types

Evaluation Study
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Blood / virology*
Child, Preschool
Desiccation*
Drug Resistance, Viral*
Genotyping Techniques / methods*
HIV Infections / virology*
HIV-1 / genetics*
HIV-1 / isolation & purification
Humans
Infant
South Africa
Specimen Handling / methods*

Abstract

Publication types

MeSH terms

Grants and funding