PEG-based ultrasound-assisted enzymatic extraction of polysaccharides from Ginkgo biloba leaves

Lijin Zhang; Shanshan Guo; Maoshan Wang; Lei He

doi:10.1016/j.ijbiomac.2015.07.023

PEG-based ultrasound-assisted enzymatic extraction of polysaccharides from Ginkgo biloba leaves

Int J Biol Macromol. 2015 Sep:80:644-50. doi: 10.1016/j.ijbiomac.2015.07.023. Epub 2015 Jul 16.

Authors

Lijin Zhang¹, Shanshan Guo¹, Maoshan Wang¹, Lei He²

Affiliations

¹ Department of Food Science and Nutrition, School of Hotel Management, University of Jinan, No. 13, Shungeng Road, Jinan, Shandong 250002, PR China.
² Department of Food Science and Nutrition, School of Hotel Management, University of Jinan, No. 13, Shungeng Road, Jinan, Shandong 250002, PR China. Electronic address: helei0508@sina.com.

PMID: 26188295
DOI: 10.1016/j.ijbiomac.2015.07.023

Abstract

In this study, one kind of environmentally friendly solvents named polyethylene glycol (PEG) was developed for the ultrasound-assisted enzymatic extraction (UAEE) of polysaccharides from Gingko biloba leaves (GBLP). Box-Behnken design (BBD) was used to optimize the UAEE conditions of GBLP. Results showed that the optimal extraction conditions were: a pH of 4.34, an extraction temperature of 51.88 °C and an extraction time of 37.13 min. Under these optimal extraction conditions, the GBLP yield was 7.29±0.21%, which was well in agreement with the value predicted by the mathematical model.

Keywords: Box–Behnken design; Ginkgo biloba leaves; Polyethylene glycol-based ultrasound-assisted enzymatic extraction; Polysaccharides; Response surface methodology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chemical Fractionation / methods*
Ginkgo biloba / chemistry*
Models, Theoretical
Plant Extracts / chemistry*
Plant Leaves / chemistry*
Polyethylene Glycols / chemistry*
Polysaccharides / chemistry*
Solvents
Sound*
Temperature

Substances

Plant Extracts
Polysaccharides
Solvents
Polyethylene Glycols