New insights into coenzyme A interaction with mercury ions provided by mass spectrometric and circular dichroism spectroscopic approaches

Claudia Andries; Marilena Manea; Gabi Drochioiu; Robert Gradinaru

doi:10.1255/ejms.1361

New insights into coenzyme A interaction with mercury ions provided by mass spectrometric and circular dichroism spectroscopic approaches

Eur J Mass Spectrom (Chichester). 2015;21(2):97-102. doi: 10.1255/ejms.1361.

Authors

Claudia Andries¹, Marilena Manea², Gabi Drochioiu³, Robert Gradinaru⁴

Affiliations

¹ Alexandru Ioan Cuza University of Iasi, Department of Chemistry, 11 Carol I, Iasi-700506, Romania.. andries.claudia17@gmail.com.
² University of Konstanz, Department of Chemistry and Zukunftskolleg, 78457 Konstanz, Germany.. marilena.manea@uni- konstanz.de.
³ Alexandru Ioan Cuza University of Iasi, Department of Chemistry, 11 Carol I, Iasi-700506, Romania.. gabidr@uaic.ro.
⁴ Alexandru Ioan Cuza University of Iasi, Department of Chemistry, 11 Carol I, Iasi-700506, Romania.. robert.gradinaru@uaic.ro.

PMID: 26181283
DOI: 10.1255/ejms.1361

Abstract

The interaction of coenzyme A (CoA) with mercury ions was investigated using electrospray ionization mass spectrometry and circular dichroism spectroscopy. Our results indicated a 1:1 stoichiometric CoA-Hg complex at physiological pH. Furthermore, the CoA conformation considerably changed in the presence of mercury ions. In addition, a by-product of the reaction, thiocoenzyme A, was identified using mass spectrometry.