Abstract
A multi-targeting protocol for the detection of three of the most important bacterial phytopathogens, based on their scientific and economic importance, was developed using an acoustic biosensor (the Quartz Crystal Microbalance) for DNA detection. Acoustic detection was based on a novel approach where DNA amplicons were monitored and discriminated based on their length rather than mass. Experiments were performed during real time monitoring of analyte binding and in a direct manner, i.e. without the use of labels for enhancing signal transduction. The proposed protocol improves time processing by circumventing gel electrophoresis and can be incorporated as a routine detection method in a diagnostic lab or an automated lab-on-a-chip system for plant pathogen diagnostics.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acoustics*
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Bacteria / isolation & purification*
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Biosensing Techniques / methods*
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Limit of Detection
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Plant Diseases / microbiology
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Plant Leaves / microbiology
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Polymerase Chain Reaction
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Solanum lycopersicum / microbiology*
Grants and funding
The European Regional Development Funds and Greek National Funds on “Competitiveness and Enterpreneurship” are acknowledged through SYNERGASIA 2011. This work was also supported by the European Commision through Framework Package 7-Information and Communication Technologies (FP7-ICT, Grant agreement No 317742) and Framework Package 7 Research Potential (REGPOT, Grant agreement No 316223). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.