Video-rate two-photon excited fluorescence lifetime imaging system with interleaved digitization

Opt Lett. 2015 Jul 15;40(14):3296-9. doi: 10.1364/OL.40.003296.

Abstract

A fast (up to video rate) two-photon excited fluorescence lifetime imaging system based on interleaved digitization is demonstrated. The system is compatible with existing beam-scanning microscopes with minor electronics and software modification. Proof-of-concept demonstrations were performed using laser dyes and biological tissue.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Image Processing, Computer-Assisted
  • Mice
  • Optical Imaging / methods*
  • Photons*
  • Tail
  • Time Factors