Concise Synthesis of Triazole-Linked 5'-Peptide-Oligonucleotide Conjugates by Click Chemistry

Qunpeng Duan; Kui Lu; Li Ma; Dongxin Zhao

doi:10.1080/15257770.2015.1037455

Concise Synthesis of Triazole-Linked 5'-Peptide-Oligonucleotide Conjugates by Click Chemistry

Nucleosides Nucleotides Nucleic Acids. 2015;34(8):579-89. doi: 10.1080/15257770.2015.1037455.

Authors

Qunpeng Duan¹, Kui Lu, Li Ma, Dongxin Zhao

Affiliation

¹ a School of Material and Chemical Engineering, Henan Institute of Engineering , Zhengzhou , China.

PMID: 26167666
DOI: 10.1080/15257770.2015.1037455

Abstract

A concise synthesis of oligonucleotide 5'-peptide-conjugates via copper(I)-catalyzed azide-alkyne 1,3-dipolar cycloaddition in aqueous solution is described. Synthesis of reagents was accomplished by on-column derivatization of corresponding peptides and oligonucleotides. This method is well suited for the preparation of peptide-oligonucleotide conjugates containing 1,2,3-triazole linkage between the 5'-position of an oligonucleotide and the N-terminus of a peptide.

Keywords: Peptides; click chemistry; conjugates; oligonucleotides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromatography, High Pressure Liquid
Click Chemistry* / methods
Hydrolysis
Oligonucleotides / chemical synthesis*
Oligonucleotides / chemistry
Peptides* / chemistry
Triazoles* / chemistry

Substances

Oligonucleotides
Peptides
Triazoles