Abstract
Piperine (P), a sensory stimulant in black pepper, is an agonist on TRPV1 receptors. Earlier work has showed capsaicin-sensitive and -insensitive mechanisms of the contractile action of P on the intestine. The current isolated organ study in the guinea-pig ileum, urinary bladder and trachea (a) confirms the presence of such components of effect (ileum and bladder); (b) indicates TRPV1 involvement in the effect of 5 or 30 µmol/l of P on the basis of an inhibitory action of the antagonist BCTC (ileum); (c) indicates that HC 030031-sensitive TRPA1 receptors and nifedipine-sensitive Ca(2+) channels contribute to the capsaicin-resistant contraction to 30 µmol/l P (ileum) and (d) shows that the contractile effect of P up to 100 µmol/l (guinea-pig trachea) or 30 µmol/l (guinea-pig urinary bladder) is capsaicin-sensitive and mediated by TRPV1 receptors/channels.
© 2015 S. Karger AG, Basel.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acetanilides / pharmacology
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Alkaloids / antagonists & inhibitors
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Alkaloids / pharmacology*
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Animals
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Benzodioxoles / antagonists & inhibitors
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Benzodioxoles / pharmacology*
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Capsaicin / pharmacology*
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Dose-Response Relationship, Drug
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Female
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Guinea Pigs
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Ileum / drug effects*
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In Vitro Techniques
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Male
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Muscle Contraction / drug effects
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Nifedipine / pharmacology
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Piperidines / antagonists & inhibitors
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Piperidines / pharmacology*
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Polyunsaturated Alkamides / antagonists & inhibitors
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Polyunsaturated Alkamides / pharmacology*
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Purines / pharmacology
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Pyrazines / pharmacology
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Pyridines / pharmacology
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TRPV Cation Channels / agonists
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TRPV Cation Channels / antagonists & inhibitors
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Trachea / drug effects*
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Urinary Bladder / drug effects*
Substances
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2-(1,3-dimethyl-2,6-dioxo-1,2,3,6-tetrahydro-7H-purin-7-yl)-N-(4-isopropylphenyl)acetamide
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Acetanilides
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Alkaloids
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Benzodioxoles
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N-(4-tert-butylphenyl)-4-(3-chloropyridin-2-yl)tetrahydropyrazine-1(2H)-carboxamide
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Piperidines
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Polyunsaturated Alkamides
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Purines
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Pyrazines
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Pyridines
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TRPV Cation Channels
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TRPV1 receptor
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Nifedipine
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Capsaicin
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piperine