STED microscopy--super-resolution bio-imaging utilizing a stimulated emission depletion

Kohei Otomo; Terumasa Hibi; Yuichi Kozawa; Tomomi Nemoto

doi:10.1093/jmicro/dfv036

STED microscopy--super-resolution bio-imaging utilizing a stimulated emission depletion

Microscopy (Oxf). 2015 Aug;64(4):227-36. doi: 10.1093/jmicro/dfv036. Epub 2015 Jul 6.

Authors

Kohei Otomo¹, Terumasa Hibi², Yuichi Kozawa³, Tomomi Nemoto⁴

Affiliations

¹ Research Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita, Sapporo 001-0020, Japan.
² Research Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita, Sapporo 001-0020, Japan Graduate School of Information Science and Technology, Hokkaido University, Kita 14 Nishi 9, Kita, Sapporo 060-0814, Japan.
³ Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Katahira 2-1-1, Aoba-ku, Sendai 980-8577, Japan.
⁴ Research Institute for Electronic Science, Hokkaido University, Kita 20 Nishi 10, Kita, Sapporo 001-0020, Japan Graduate School of Information Science and Technology, Hokkaido University, Kita 14 Nishi 9, Kita, Sapporo 060-0814, Japan tn@es.hokudai.ac.jp.

PMID: 26152214
DOI: 10.1093/jmicro/dfv036

Abstract

One of the most popular super-resolution microscopies that breaks the diffraction barrier is stimulated emission depletion (STED) microscopy. As the optical set-up of STED microscopy is based on a laser scanning microscopy (LSM) system, it potentially has several merits of LSM like confocal or two-photon excitation LSM. In this article, we first describe the principles of STED microscopy and then describe the features of our newly developed two-photon excitation STED microscopy. On the basis of our recent results and those of other researchers, we conclude by discussing future research and new technologies in this field.

Keywords: liquid crystal devices; optical vortex; two-photon microscopy.

Publication types

Research Support, Non-U.S. Gov't